WEKO3
アイテム
{"_buckets": {"deposit": "071c9a0e-da73-4066-8125-7e7116ce7582"}, "_deposit": {"id": "49112", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "49112"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00049112", "sets": ["857", "2679", "2556"]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "2802", "bibliographicPageStart": "2793", "bibliographicVolumeNumber": "30", "bibliographic_titles": [{"bibliographic_title": "Journal of Applied Phycology"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Lipid droplets are ubiquitous cellular compartments that store neutral lipids and specific proteins localize on their surface. These proteins work as a scaffold in maintaining the lipid droplet structure or as regulators of lipogenesis or lipolysis. Previously, the most abundant lipid droplet protein, namely stramenopile-type lipid droplet protein (StLDP), was identified in the marine diatom Phaeodactylum tricornutum; however, its function remains unclear because StLDP does not reveal homology with known lipid droplet proteins and lacks a predictable domain. In this study, P. tricornutum was transformed to express a homologous StLDP gene under an fcpA promoter in order to determine its function. StLDP expression was strongly enhanced in the mutant (H8), especially in nitrogen-sufficient conditions; however, it was attenuated in nitrogen-deficient conditions. Despite the strong expression, no significant difference was observed in the lipid composition between the wild type (WT) and H8 under nitrogen-sufficient conditions. After cultivation in nitrogen-free medium for 6 days, neutral lipid content significantly increased in H8 than in WT. After 2 days of cultivation in nitrogen-free medium, 97.0% of single cells in WT formed one or two lipid droplets, whereas in H8, this proportion decreased to 78.8%, and the proportion of cells forming three or four lipid droplets increased. Thus, the function of StLDP was speculated to sequester triacylglycerol on the initial lipid droplet formation.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Springer Netherlands"}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1007/s10811-018-1402-9", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This is a pre-print of an article published in Journal of Applied Phycology. The final authenticated version is available online at: https://doi.org/10.1007/s10811-018-1402-9"}]}, "item_5_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0921-8971", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10729877 ", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NIIサブジェクト", "attribute_value_mlt": [{"subitem_subject": "生物学", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "吉田, 昌樹"}, {"creatorName": "ヨシダ, マサキ", "creatorNameLang": "ja-Kana"}, {"creatorName": "YOSHIDA, Masaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "106760", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "10449308", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000010449308"}, {"nameIdentifier": "0000001222", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001222"}]}, {"creatorNames": [{"creatorName": "鈴木, 石根"}, {"creatorName": "スズキ, イワネ", "creatorNameLang": "ja-Kana"}, {"creatorName": "SUZUKI, Iwane", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "438", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "10290909", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000010290909"}, {"nameIdentifier": "0000001196", "nameIdentifierScheme": "筑波大学研究者総覧", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001196"}]}, {"creatorNames": [{"creatorName": "Yoneda, Kohei", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "205766", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Makoto M.", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "205767", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-10-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "JAP_30-5-2793.pdf", "filesize": [{"value": "2.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2700000.0, "url": {"label": "JAP_30-5-2793", "url": "https://tsukuba.repo.nii.ac.jp/record/49112/files/JAP_30-5-2793.pdf"}, "version_id": "58e16cd0-9092-45b8-b20e-ed64008561f7"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Homologous expression of lipid droplet protein-enhanced neutral lipid accumulation in the marine diatom Phaeodactylum tricornutum", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Homologous expression of lipid droplet protein-enhanced neutral lipid accumulation in the marine diatom Phaeodactylum tricornutum"}]}, "item_type_id": "5", "owner": "1", "path": ["857", "2679", "2556"], "permalink_uri": "http://hdl.handle.net/2241/00154584", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-02-13"}, "publish_date": "2019-02-13", "publish_status": "0", "recid": "49112", "relation": {}, "relation_version_is_last": true, "title": ["Homologous expression of lipid droplet protein-enhanced neutral lipid accumulation in the marine diatom Phaeodactylum tricornutum"], "weko_shared_id": 5}
Homologous expression of lipid droplet protein-enhanced neutral lipid accumulation in the marine diatom Phaeodactylum tricornutum
http://hdl.handle.net/2241/00154584
http://hdl.handle.net/2241/0015458480754b94-cd09-421c-a7cf-7fafc9ff2cfc
名前 / ファイル | ライセンス | アクション |
---|---|---|
JAP_30-5-2793 (2.7 MB)
|
|
Item type | Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-02-13 | |||||
タイトル | ||||||
タイトル | Homologous expression of lipid droplet protein-enhanced neutral lipid accumulation in the marine diatom Phaeodactylum tricornutum | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
吉田, 昌樹
× 吉田, 昌樹× 鈴木, 石根× Yoneda, Kohei× Watanabe, Makoto M. |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Lipid droplets are ubiquitous cellular compartments that store neutral lipids and specific proteins localize on their surface. These proteins work as a scaffold in maintaining the lipid droplet structure or as regulators of lipogenesis or lipolysis. Previously, the most abundant lipid droplet protein, namely stramenopile-type lipid droplet protein (StLDP), was identified in the marine diatom Phaeodactylum tricornutum; however, its function remains unclear because StLDP does not reveal homology with known lipid droplet proteins and lacks a predictable domain. In this study, P. tricornutum was transformed to express a homologous StLDP gene under an fcpA promoter in order to determine its function. StLDP expression was strongly enhanced in the mutant (H8), especially in nitrogen-sufficient conditions; however, it was attenuated in nitrogen-deficient conditions. Despite the strong expression, no significant difference was observed in the lipid composition between the wild type (WT) and H8 under nitrogen-sufficient conditions. After cultivation in nitrogen-free medium for 6 days, neutral lipid content significantly increased in H8 than in WT. After 2 days of cultivation in nitrogen-free medium, 97.0% of single cells in WT formed one or two lipid droplets, whereas in H8, this proportion decreased to 78.8%, and the proportion of cells forming three or four lipid droplets increased. Thus, the function of StLDP was speculated to sequester triacylglycerol on the initial lipid droplet formation. | |||||
書誌情報 |
Journal of Applied Phycology 巻 30, 号 5, p. 2793-2802, 発行日 2018-10 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0921-8971 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10729877 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s10811-018-1402-9 | |||||
権利 | ||||||
権利情報 | This is a pre-print of an article published in Journal of Applied Phycology. The final authenticated version is available online at: https://doi.org/10.1007/s10811-018-1402-9 | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Springer Netherlands |