Lethal and sublethal effects of naphthalene and 1,2-dimethylnaphthalene on the marine copepod Paracartia grani

Abstract

Here we evaluate the effects of two quantitatively very important components of the water soluble fractions of fuel oils (naphthalene and 1,2-dimethylnaphthalene, hereafter NAPH and C2-NAPH, respectively) on the survival, feeding and egg production rates, and viability of eggs of the coastal copepod Paracartia (Acartia) grani. Acute toxicity responses resulted in lethal concentrations (LC50) of 2,535 and 161 μg l⁻¹ for NAPH and C2-NAPH, respectively, with no evidence of narcotic effects. Hydrocarbon-specific differences in the toxicity response indicate that sublethal effects (EC50) on feeding by C2-NAPH were likely driven by induced mortality, whereas NAPH has direct negative effects on feeding. Sublethal effects on egg production rates followed a similar detrimental pattern to the one exhibited by feeding rates, suggesting that the lower egg production rates were mediated by the decrease in feeding rates. At the exposure time tested (24 h), the 50% reduction effective concentrations (EC50) determined for sublethal effects were relatively high in comparison with hydrocarbons’ concentrations found under natural circumstances. Long exposure (4 days) of P. grani adults to the tested hydrocarbons at concentrations well below the recorded EC50, however, had no significant effects on feeding, egg production and hatching rates. The viability of the eggs was either not affected or only slightly influenced when healthy eggs were incubated under very high concentrations (up to 6,400 and 700 μg l⁻¹ NAPH and C2-NAPH, respectively). The significance of the effects of oil spills on marine zooplankton communities is discussed in light of the results presented in this study.