Abstract
Although microsatellites are the most popular molecular markers for population genetic studies, only a few loci have been characterized for sponges (Porifera). In the present work, we describe the isolation and characterization of seven polymorphic microsatellite loci from an enriched library of the marine calcareous sponge Clathrina aurea. This species is considered endemic to Brazil, where it is widely distributed. However, it was recently found in Peruvian waters. Seven loci were tested in 30 individuals of one population in southeastern Brazil (Cabo Frio). The number of alleles ranged from 5 to 18 (average 12.3 ± 3.9). The species presented high average values of observed and expected heterozygosities: 0.672 ± 0.25 and 0.837 ± 0.08, respectively. No linkage disequilibrium between all pairs of loci was found, but two loci in this population showed a significant excess of homozygotes. This is the second set of loci ever characterized for a calcareous sponge. These loci will be useful for population genetics studies among Brazilian populations, to elucidate the possible introduction of this species in the Pacific Ocean and to analyze other still poorly investigated issues in sponges.
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Acknowledgements
We thank Lívia Moura, Camila Mantello and Anete Pereira de Souza from the Universidade Estadual de Campinas (UniCamp) for helping in the genomic DNA purification and in the construction of the microsatellite enriched library, and Antonio Solé-Cava for the use of his laboratory during the completion of microsatellite development and optimization; Emilio Lanna, Eduardo Resende and the staff of LitoralSub/ Cabo Frio for scuba diving support; Carla Zilberberg for helping in the DNA extraction and Carlos Guerra Schrago for helping in the analyses. Sponge specimens were sampled with permission of the Brazilian authorities (IBAMA and ICMBIO). This study was funded by the Rio de Janeiro State Research Foundation (Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro – FAPERJ). M.K. received a research fellowship from the Brazilian National Council of Technological and Scientific Development (CNPq). A.P. and F.F.C. received scholarships from the CNPq and FAPERJ, respectively, and H.C. received a post-doc fellowship from CNPq. This work was part of the Master Thesis of A.P. presented to the Graduation Program in Zoology of the Museu Nacional do Rio de Janeiro/UFRJ.
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Padua, A., Cavalcanti, F.F., Cunha, H. et al. Isolation and characterization of polymorphic microsatellite loci from Clathrina aurea (Porifera, Calcarea). Mar Biodiv 43, 489–492 (2013). https://doi.org/10.1007/s12526-013-0167-2
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DOI: https://doi.org/10.1007/s12526-013-0167-2