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Study of microbial communities in redox zone of meromictic lakes isolated from the White Sea using spectral and molecular methods

Published online by Cambridge University Press:  01 July 2015

E.D. Krasnova ,
A.V. Kharcheva ,
I.A. Milyutina ,
D.A. Voronov  and
S.V. Patsaeva Open the ORCID record for S.V. Patsaeva [Opens in a new window]
E.D. Krasnova*
Affiliation:
Biological Faculty of Lomonosov Moscow State University, Leninskye gory, 1, bld. 12, 119234 Moscow, Russia
A.V. Kharcheva
Affiliation:
Faculty of Physics, Lomonosov Moscow State University, Leninskye gory, 1, bld. 2, Moscow 119991, Russia
I.A. Milyutina
Affiliation:
A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskye gory, 1, bld. 40, Moscow 119992, Russia
D.A. Voronov
Affiliation:
A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskye gory, 1, bld. 40, Moscow 119992, Russia A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Bolshoy Karetny per. 19, Moscow 127994, Russia
S.V. Patsaeva
Affiliation:
Faculty of Physics, Lomonosov Moscow State University, Leninskye gory, 1, bld. 2, Moscow 119991, Russia
*
Correspondence should be addressed to: E.D. Krasnova, Leninskye gory, 1, bld. K, apt. 131, Moscow, 119234, Russia email: e_d_krasnova@wsbs-msu.ru
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Abstract

Due to postglacial isostatic uplift many stratified lakes, at different stages of isolation, are located along the shores of the White Sea. In five lakes, located near the White Sea Biological Station of Moscow State University, salinity, temperature, pH, concentration of dissolved oxygen, redox potential, and illuminance were measured. Distribution of microorganisms and spectral properties of water layers were also studied. All the lakes had a narrow bright coloured layer in the redox zone caused by mass development of phototropic microorganisms. Light absorption and fluorescence spectra indicated algae containing chlorophyll a predominate in the red water layers while the colouration of green and brown layers is caused by green sulphur bacteria with bacteriochlorophylls d and e. Sunlight is completely absorbed in the redox zone because of the high density of algae and/or bacteria, resulting in aphotic conditions below. Coloured layers act as a specific biotope for special communities of microorganisms. Eukaryotes identified by the 18S rRNA gene included different species of mixotrophic algae and ciliates resistant to anoxia. The water layer colour and spectral characteristics (i.e. light absorption and fluorescence) of water in the redox zone can be considered indicators of the stage of lake isolation from the sea, with the red colour caused by cryptophyte alga Rhodomonas sp. bloom found in earlier stages and brown and green colours caused by green sulphur bacteria in later stages.

Keywords

Meromictic lakechemoclineredox zoneabsorption and fluorescence spectrachlorophyllbacteriochlorophyllmicrobial communityWhite Sea
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 95 , Issue 8 , December 2015 , pp. 1579 - 1590
Copyright
Copyright © Marine Biological Association of the United Kingdom 2015 

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