Genomic characterization of an endemic Mycobacterium tuberculosis strain: Evolutionary and epidemiologic implications
Date
2004
Authors
Nguyen D.
Brassard P.
Menzies D.
Thibert L.
Warren R.
Mostowy S.
Behr M.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
In a study of 302 Mycobacterium tuberculosis clinical isolates from the low-incidence Canadian-born population of Quebec, we characterized a large endemic strain family by using genomic deletions. The DS6Quebec deleted region (11.4 kb) defined a strain family of 143 isolates encompassing two subgroups: one characterized by pyrazinamide (PZA) susceptibility and the other marked by a PZA-monoresistant phenotype. A second deletion (8 bp) in the pncA gene was shared by all 76 isolates with the PZA resistance phenotype, whereas a third DRv0961 deletion (970 bp) defined a further subset of 15 isolates. From their deletion profiles, we derived a most parsimonious evolutionary scenario and compared multiple standard genotyping modalities (using IS6110 restriction fragment length polymorphism [RFLP], spoligotyping, and mycobacterial interspersed repetitive units [MIRU]) across the deletion-based subgroups. The use of a single genotyping modality yielded an unexpectedly high proportion of clustered isolates for a high IS6110 copy strain (27% by IS6110 RFLP, 61% by MIRU, and 77% by spoligotyping). By combining all three modalities, only 14% were genotypically clustered overall, a result more congruent with the epidemiologic profile of reactivation tuberculosis, as suggested by the older age (mean age, 60 years), rural setting, and low proportion of epidemiologic links. These results provide insight into the evolution of genotypes in endemic strains and the potential for false clustering in molecular epidemiologic studies.
Description
Keywords
genomic DNA, pyrazinamide, tuberculostatic agent, adult, antibiotic sensitivity, article, bacterial genome, Canada, endemic disease, female, gene, gene mutation, genotype, human, major clinical study, male, minimum inhibitory concentration, Mycobacterium, Mycobacterium tuberculosis, nonhuman, nucleotide sequence, phenotype, pnca gene, polymerase chain reaction, priority journal, restriction fragment length polymorphism, strain difference, tuberculosis, Evolution, Genome, Bacterial, Genotype, Mycobacterium tuberculosis, Polymorphism, Restriction Fragment Length, Repetitive Sequences, Nucleic Acid, Actinobacteria (class), Animalia, Bacteria (microorganisms), Mycobacterium, Mycobacterium tuberculosis, Prokaryota, uncultured actinomycete
Citation
Journal of Clinical Microbiology
42
6
42
6