Rotenone induces degeneration of photoreceptors and impairs the dopaminergic system in the rat retina
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Título: | Rotenone induces degeneration of photoreceptors and impairs the dopaminergic system in the rat retina |
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Autor/es: | Esteve Rudd, Julián | Fernández-Sánchez, Laura | Lax, Pedro | Juan Navarro, Emilio de | Martín-Nieto, José | Cuenca, Nicolás |
Grupo/s de investigación o GITE: | Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS) | Genética Humana y de Mamíferos (GHM) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | Retina | Parkinson disease | Rotenone | Retinal degeneration | Pesticide | Mitochondria | Photoreceptor | Dopaminergic neuron |
Área/s de conocimiento: | Biología Celular | Fisiología | Genética |
Fecha de publicación: | 25-jun-2011 |
Editor: | Elsevier |
Cita bibliográfica: | ESTEVE-RUDD, Julián, et al. “Rotenone induces degeneration of photoreceptors and impairs the dopaminergic system in the rat retina”. Neurobiology of Disease. Vol. 44, Issue 1 (Oct. 2011). ISSN 0969-9961, pp. 102-115 |
Resumen: | Rotenone is a widely used pesticide and a potent inhibitor of mitochondrial complex I (NADH-quinone reductase) that elicits the degeneration of dopaminergic neurons and thereby the appearance of a parkinsonian syndrome. Here we have addressed the alterations induced by rotenone at the functional, morphological and molecular levels in the retina, including those involving both dopaminergic and non-dopaminergic retinal neurons. Rotenone-treated rats showed abnormalities in equilibrium, postural instability and involuntary movements. In their outer retina we observed a loss of photoreceptors, and a reduced synaptic connectivity between those remaining and their postsynaptic neurons. A dramatic loss of mitochondria was observed in the inner segments, as well as in the axon terminals of photoreceptors. In the inner retina we observed a decrease in the expression of dopaminergic cell molecular markers, including loss of tyrosine hydroxylase immunoreactivity, associated with a reduction of the dopaminergic plexus and cell bodies. An increase in immunoreactivity of AII amacrine cells for parvalbumin, a Ca2+-scavenging protein, was also detected. These abnormalities were accompanied by a decrease in the amplitude of scotopic and photopic a- and b-waves and an increase in the b-wave implicit time, as well as by a lower amplitude and greater latency in oscillatory potentials. These results indicate that rotenone induces loss of vision by promoting photoreceptor cell death and impairment of the dopaminergic retinal system. |
Patrocinador/es: | This research was supported by grants from the Spanish Ministerio de Ciencia e Innovación (MICIN; BFU2009-07793/BFI), Instituto de Salud Carlos III, (RETICS RD07/0062/0012), Organización Nacional de Ciegos de España (ONCE), Fundación Lucha contra la Ceguera (FUNDALUCE) and Fundación Médica Mutua Madrileña. |
URI: | http://hdl.handle.net/10045/25069 |
ISSN: | 0969-9961 (Print) | 1095-953X (Online) |
DOI: | 10.1016/j.nbd.2011.06.009 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/j.nbd.2011.06.009 |
Aparece en las colecciones: | INV - NEUROVIS - Artículos de Revistas INV - GHM - Artículos de Revistas |
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Archivo | Descripción | Tamaño | Formato | |
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2011_Esteve_etal_ND.pdf | Versión final (acceso restringido) | 3,75 MB | Adobe PDF | Abrir Solicitar una copia |
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