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Decreased levels of autoantibody against histone deacetylase 3 in patients with systemic sclerosis.
http://hdl.handle.net/10069/23058
http://hdl.handle.net/10069/23058c5506f71-5bef-4b34-a144-e55552b64d66
名前 / ファイル | ライセンス | アクション |
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Auto42_120.pdf (226.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-03-11 | |||||
タイトル | ||||||
タイトル | Decreased levels of autoantibody against histone deacetylase 3 in patients with systemic sclerosis. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | systemic sclerosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HDAC-3 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | autoantibody | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kuwatsuka, Yutaka
× Kuwatsuka, Yutaka× Ogawa, Fumihide× Iwata, Yohei× Komura, Kazuhiro× Muroi, Eiji× Hara, Toshihide× Takenaka, Motoi× Shimizu, Kazuhiro× Hasegawa, Minoru× Fujimoto, Manabu× Sato, Shinichi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Systemic sclerosis (SSc) is characterized by immunological abnormalities, especially the production of autoantibodies against various cellular components. Treatment with histone deacetylase (HDAC) inhibitors prevents collagen accumulation in a mouse SSc model. Additionally, autoantibody against HDAC-3 is produced in colon cancer patients, while HDAC-1 and HDAC-2 do not elicit autoantibody response. To determine the presence and levels of antibodies (Abs) against HDAC-3 in SSc. Anti-HDAC-3 Ab was examined by enzyme-linked immunosorbent assay (ELISA) and immunoblotting using human recombinant HDAC-3. The HDAC-3 activity was evaluated by ELISA using the fluorimetric HDAC lysyl substrate that comprises an acetylated lysine side chain. Contrary to our hypothesis that autoimmune background in SSc induced the production of autoantibody against HDACs, IgG and IgM anti-HDAC-3 Ab levels in SSc patients were significantly lower than in normal controls (p < 0.0005 and 0.001, respectively). Furthermore, decreased levels of IgG anti-HDAC-3 Ab were specific to SSc, since IgG anti-HDAC-3 Ab levels in patients with dermatomyositis (DM) and those with systemic lupus erythematosus (SLE) were similar and slightly increased relative to normal controls, respectively. Immunoblotting analysis showed that anti-HDAC-3 Ab was detected in normal controls and patients with DM or SLE, while it was absent in SSc patients. The HDAC-3 activity was significantly inhibited by IgG isolated from sera of normal controls, whereas such inhibitory effect was not observed by IgG isolated from sera of SSc patients. These results indicate the lack of anti-HDAC-3 autoantibody in SSc patients, which is produced in healthy individuals as well as DM and SLE patients, suggesting that this autoantibody might function as protective Ab. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | This is an electronic version of an article published in Free Radical Research, 42(11-12), 957-965: 2008 November. Free Radical Research is available online at: http://informahealthcare.com/doi/abs/10.1080/08916930802406300 | |||||
書誌情報 |
Autoimmunity 巻 42, 号 2, p. 120-125, 発行日 2009-02 |
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出版者 | ||||||
出版者 | Taylor & Francis | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1607842X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10707648 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19021012 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1080/08916930802406300 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Autoimmunity, 42(2), pp.120-125; 2009 |