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In Situ Localization of Gene-Specific Transcription Regulatory Factors by Southwestern Histochemistry (<Symposium XII>Hybridohistochemistry)
http://hdl.handle.net/10069/23713
http://hdl.handle.net/10069/237139fc0fdd3-71d5-4ffe-841a-36868e519abb
名前 / ファイル | ライセンス | アクション |
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AHC32_255.pdf (4.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-08-31 | |||||
タイトル | ||||||
タイトル | In Situ Localization of Gene-Specific Transcription Regulatory Factors by Southwestern Histochemistry (<Symposium XII>Hybridohistochemistry) | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Southwestern histochemistry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transcription factors | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nonradioactive | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Double-stranded DNA probe | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Immunohistochemistry | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Koji, Takehiko
× Koji, Takehiko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To understand better the regulation of gene expression, an analysis of the expression of trans-acting transcription regulatory factors in individual cells is essential. Although cellular analysis of specific mRNA expression is successfully performed by in situ hybridization, the results are not enough to tell whether an elevated level of certain mRNA is due to an increased rate of transcription of the gene or due to a decreased rate of degradation of the mRNA. Recently, we have developed a new method, Southwestern histochemistry, which allows us to localize transcription regulatory factors in situ. In principle, double-stranded (ds) DNA harboring specific response element base sequences is synthesized and labeled with a hapten such as thymine-thymine (T-T) dimer and digoxigenin (Dig). After the reaction of frozen tissue sections with the probe ds DNA, the signal is detected enzyme-immunohistochemically by horseradish peroxidase (HRP) -labeled anti-T-T dimer or anti-Dig, respectively. As a model system, we localized estrogen receptor (ER) in mouse uterine tissue sections by immunohistochemistry with 1D5 monoclonal antibody and Southwestern histochemistry using T-T dimerized estrogen responsive element (ERE) ds DNA of vitellogenin. Consequently, very similar localization of ER was obtained by both methods. This result, together with other examples, further indicates the usefulness of Southwestern histochemistry as a method to localize specific transcription regulatory factors in situ. | |||||
書誌情報 |
Acta Histochemica et Cytochemica 巻 32, 号 3, p. 255-260, 発行日 1999 |
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出版者 | ||||||
出版者 | The Japan Society of Histochemistry and Cytochemistry | |||||
出版者別言語 | ||||||
日本組織細胞化学会 | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00445991 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475800 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508022 | |||||
権利 | ||||||
権利情報 | Copyright ©1999 By the Japan Society of Histochemistry and Cytochemistry | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta Histochemica et Cytochemica, 32(3), pp.255-260; 1999 |