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Effects of tricalcium silicate cements on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells in vitro
http://hdl.handle.net/10069/34684
http://hdl.handle.net/10069/34684c5ee5d54-5cd7-4073-bca8-230dbbe2c090
名前 / ファイル | ライセンス | アクション |
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ActaBio10_3327.pdf (626.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-08-25 | |||||
タイトル | ||||||
タイトル | Effects of tricalcium silicate cements on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells in vitro | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bone marrow | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human origin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mesenchymal stem cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteogenic differentiation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tricalcium silicate cements | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Eid, Ashraf A.
× Eid, Ashraf A.× Hussein, Khaled A.× Niu, Li-na× Li, Guo-hua× Watanabe, Ikuya× Al-Shabrawey, Mohamed× Pashley, David H.× Tay, Franklin R. |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Tricalcium silicate cements have been successfully employed in the biomedical field as bioactive bone and dentin substitutes, with widely acclaimed osteoactive properties. This research analyzed the effects of different tricalcium silicate cement formulations on the temporal osteoactivity profile of human bone marrow-derived mesenchymal stem cells (hMW-MSCs). These cells were exposed to four commercially available tricalcium silicate cement formulations in osteogenic differentiation medium. After 1, 3, 7 and 10 days, quantitative real-time polymerase chain reaction and Western blotting were performed to detect expression of the target osteogenic markers ALP, RUNX2, OSX, OPN, MSX2 and OCN. After 3, 7, 14 and 21 days, alkaline phosphatase assay was performed to detect changes in intracellular enzyme level. An Alizarin Red S assay was performed after 28 days to detect extracellular matrix mineralization. In the presence of tricalcium silicate cements, target osteogenic markers were downregulated at the mRNA and protein levels at all time points. Intracellular alkaline phosphatase enzyme levels and extracellular mineralization of the experimental groups were not significantly different from the untreated control. Quantitative polymerase chain reaction results showed increases in downregulation of RUNX2, OSX, MSX2 and OCN with increasing time of exposure to the tricalcium silicate cements, while ALP showed peak downregulation at day 7. For Western blotting, OSX, OPN, MSX2 and OCN showed increased downregulation with increased exposure time to the tested cements. Alkaline phosphatase enzyme levels generally declined after day 7. Based on these results, it is concluded that tricalcium silicate cements do not induce osteogenic differentiation of hBM-MSCs in vitro. | |||||
書誌情報 |
Acta Biomaterialia 巻 10, 号 7, p. 3327-3334, 発行日 2014-07 |
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出版者 | ||||||
出版者 | Elsevier BV | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 17427061 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.actbio.2014.04.006 | |||||
権利 | ||||||
権利情報 | c 2014 Published by Elsevier Ltd. on behalf of Acta Materialia Inc. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Acta Biomaterialia. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Acta Biomaterialia, 10, 7, (2014) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta Biomaterialia, 10(7), pp.3327-3334; 2014 |