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Nitrogen fixation and nifH diversity in human gut microbiota
http://hdl.handle.net/10069/37060
http://hdl.handle.net/10069/3706004917723-1120-433d-8e90-9167840f1566
名前 / ファイル | ライセンス | アクション |
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ISYO47_Igai.pdf (1.5 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2017-02-27 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Nitrogen fixation and nifH diversity in human gut microbiota | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
猪飼, 桂
× 猪飼, 桂 |
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著者別名 | ||||||
姓名 | Igai, Katsura | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | It has been hypothesized that nitrogen fixation occurs in the human gut. However, whether the gut microbiota truly has this potential remains unclear. We investigated the nitrogen-fixing activity and diversity of the nitrogenase reductase (NifH) genes in the faecal microbiota of humans, focusing on Papua New Guinean and Japanese individuals with low to high habitual nitrogen intake. A 15N2 incorporation assay showed significant enrichment of 15N in all faecal samples, irrespective of the host nitrogen intake, which was also supported by an acetylene reduction assay. The fixed nitrogen corresponded to 0.01% of the standard nitrogen requirement for humans, although our data implied that the contribution in the gut in vivo might be higher than this value. The nifH genes recovered in cloning and metagenomic analyses were classified in two clusters: one comprising sequences almost identical to Klebsiella sequences and the other related to sequences of Clostridiales members. These results are consistent with an analysis of databases of faecal metagenomes from other human populations. Collectively, the human gut microbiota has a potential for nitrogen fixation, which may be attributable to Klebsiella and Clostridiales strains, although no evidence was found that the nitrogen-fixing activity substantially contributes to the host nitrogen balance. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)乙第47号 学位授与年月日:平成29年2月22日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Katsura Igai, Manabu Itakura, Suguru Nishijima, Hirohito Tsurumaru, Wataru Suda, Takumi Tsutaya, Eriko Tomitsuka, Kiyoshi Tadokoro, Jun Baba, Shingo Odani, Kazumi Natsuhara, Ayako Morita, Minoru Yoneda, Andrew R. Greenhill, Paul F. Horwood, Jun-ichi Inoue, Moriya Ohkuma, Yuichi Hongoh, Taro Yamamoto, Peter M. Siba, Masahira Hattori, Kiwamu Minamisawa & Masahiro Umezaki | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Scientific Reports, 6, 31942; 2016 | |||||
書誌情報 |
Scientific Reports 巻 6, p. 31942, 発行日 2017-02-22 |
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EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 20452322 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/srep31942 | |||||
権利 | ||||||
権利情報 | c The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | ヒト腸内微生物叢における窒素固定活性とnifH遺伝子の多様性 | |||||
出版者 | ||||||
出版者 | Springer Nature | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2017-02-22 | |||||
学位授与番号 | ||||||
学位授与番号 | 乙医歯薬第47号 | |||||
学位の種類 | ||||||
論文博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2017-02-22) |