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Immunogenicity of Japanese Encephalitis Virus Envelope Glycoprotein E Prepared by Four Different Methods
http://hdl.handle.net/10069/4568
http://hdl.handle.net/10069/456846f8e188-493f-4c19-aacc-df86de33a486
名前 / ファイル | ライセンス | アクション |
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tm32_03_02_t.pdf (1.2 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-04-26 | |||||
タイトル | ||||||
タイトル | Immunogenicity of Japanese Encephalitis Virus Envelope Glycoprotein E Prepared by Four Different Methods | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Srivastava, Ashok Kumar
× Srivastava, Ashok Kumar× Morita, Kouichi× Igarashi, Akira |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Envelope glycoprotein (E) of Japanese encephalitis (JE) virus was prepared from purified virion by Triton X-100 (TX-100) treatment (E-TX), polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulphate (SDS) after reduction (E-2ME-SDS) or without reduction (E-IAA-SDS), and high performance liquid chromatography after denaturation with guanidine hydrochloride (E-HPLC). E-2ME-SDS, E-IAA-SDS and particularly E-HPLC were less immunogenic than E-TX, complete virion or formalin-inactivated JE vaccine, which were almost similarly immunogenic to produce anti-JE ELISA and neutralizing (N) antibodies. E-HPLC produced low but definite N antibodies with highest N/ELISA ratio among all immunogens tested. The result indicated that most of the JE virus E protein N and ELISA epitopes were conformational, but some N epitope(s) were resistant to denaturation and could be preserved in E-HPLC better than the ELISA epitopes. | |||||
書誌情報 |
熱帯医学 Tropical medicine 巻 32, 号 3, p. 103-113, 発行日 1990-09-28 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855643 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00199644 | |||||
出版者 | ||||||
出版者 | 長崎大学熱帯医学研究所 | |||||
出版者別言語 | ||||||
Institute of Tropical Medicine, Nagasaki University | ||||||
sortkey | ||||||
P00103-P00113 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 熱帯医学 Tropical medicine 32(3). p103-113, 1990 |