Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis
Author(s)
Hare, Kim M
Marsh, Robyn L
Binks, Michael J
Grimwood, Keith
Pizzutto, Susan J
Leach, Amanda J
Chang, Anne B
Smith-Vaughan, Heidi C
Griffith University Author(s)
Year published
2013
Metadata
Show full item recordAbstract
Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.
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Journal Title
Journal of Microbiological Methods
Volume
92
Subject
Microbiology
Cardiovascular medicine and haematology not elsewhere classified
Medical microbiology