WEKO3
アイテム
{"_buckets": {"deposit": "c31ecbbf-7ec7-426a-af89-4ec96bbbdaf9"}, "_deposit": {"created_by": 1, "id": "22434", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "22434"}, "status": "published"}, "_oai": {"id": "oai:u-fukui.repo.nii.ac.jp:00022434", "sets": ["2404"]}, "author_link": ["64813", "64814", "64812", "64811", "64816", "64817", "64815", "64810"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1997-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "145", "bibliographicPageStart": "137", "bibliographicVolumeNumber": "197", "bibliographic_titles": [{"bibliographic_title": "Gene -Amsterdam-"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Lactobacillus plantarum phage φg1e has two consecutive cell lysis genes hol–lys (Oki et al., 1996b). In the present study, functional and structural properties of the hol protein (Hol) were characterized in Escherichia coli. Electron microscopic examinations showed that hol under plac in E. coli XL1-Blue injured the inner membrane to yield empty ghost cells with the bulk of the cell wall undisturbed. Northern blot analysis indicated that hol–lys genes under plac were co-transcribed, although the amount of hol transcript was larger than that of lys, ceasing via an apparently ρ-independent terminator just downstream of hol. However, deletion and/or fusion experiments suggested that: (1) the N-terminal half of Φg1e Hol composed of three putative transmembrane domains may be responsible for interaction with membrane; (2) the N-terminal end (five amino acids) seems nonessential; and (3) the C-terminal half containing charged amino acids appears to be involved in proper hol function. These results suggest that Φg1e Hol is a member of the lambdoid holin family, but divergent in several properties from lambda holin.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier Science B.V."}]}, "item_10001_relation_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "TD00006847", "subitem_relation_type_select": "NCID"}}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "3781119", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_b1a7d7d4d402bcce", "subitem_version_type": "AO"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "OKI, Masaya"}], "nameIdentifiers": [{"nameIdentifier": "64810", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KAKIKAWA, Makiko"}], "nameIdentifiers": [{"nameIdentifier": "64811", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "NAKAMURA, Shogo"}], "nameIdentifiers": [{"nameIdentifier": "64812", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YAMAMURA, Ei-Tora"}], "nameIdentifiers": [{"nameIdentifier": "64813", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "WATANABE, Kouichi"}], "nameIdentifiers": [{"nameIdentifier": "64814", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SASAMOTO, Masae"}], "nameIdentifiers": [{"nameIdentifier": "64815", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TAKETO, Akira"}], "nameIdentifiers": [{"nameIdentifier": "64816", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KODAIRA, Ken-ichi"}], "nameIdentifiers": [{"nameIdentifier": "64817", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-08-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "BD00006847_001.pdf", "filesize": [{"value": "2.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2000000.0, "url": {"label": "BD00006847_001.pdf", "url": "https://u-fukui.repo.nii.ac.jp/record/22434/files/BD00006847_001.pdf"}, "version_id": "f9a6001f-18d7-4c3f-b984-1220b7a39acf"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Bacteriophage Φg1e", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Deletion", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fusion", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Holin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Transmembrane", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "other", "resourceuri": "http://purl.org/coar/resource_type/c_1843"}]}, "item_title": "Functional and structural features of the holin HOL protein of the Lactobacillus plantarum phage Φg1e: analysis in Escherichia coli system", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Functional and structural features of the holin HOL protein of the Lactobacillus plantarum phage Φg1e: analysis in Escherichia coli system"}]}, "item_type_id": "10001", "owner": "1", "path": ["2404"], "permalink_uri": "http://hdl.handle.net/10098/1623", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-02-29"}, "publish_date": "2008-02-29", "publish_status": "0", "recid": "22434", "relation": {}, "relation_version_is_last": true, "title": ["Functional and structural features of the holin HOL protein of the Lactobacillus plantarum phage Φg1e: analysis in Escherichia coli system"], "weko_shared_id": 1}
Functional and structural features of the holin HOL protein of the Lactobacillus plantarum phage Φg1e: analysis in Escherichia coli system
http://hdl.handle.net/10098/1623
http://hdl.handle.net/10098/162388c77eeb-30fa-468f-9df3-1e8dff0777b2
名前 / ファイル | ライセンス | アクション |
---|---|---|
BD00006847_001.pdf (2.0 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2008-02-29 | |||||
タイトル | ||||||
タイトル | Functional and structural features of the holin HOL protein of the Lactobacillus plantarum phage Φg1e: analysis in Escherichia coli system | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bacteriophage Φg1e | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Deletion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fusion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Holin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transmembrane | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_1843 | |||||
資源タイプ | other | |||||
著者 |
OKI, Masaya
× OKI, Masaya× KAKIKAWA, Makiko× NAKAMURA, Shogo× YAMAMURA, Ei-Tora× WATANABE, Kouichi× SASAMOTO, Masae× TAKETO, Akira× KODAIRA, Ken-ichi |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Lactobacillus plantarum phage φg1e has two consecutive cell lysis genes hol–lys (Oki et al., 1996b). In the present study, functional and structural properties of the hol protein (Hol) were characterized in Escherichia coli. Electron microscopic examinations showed that hol under plac in E. coli XL1-Blue injured the inner membrane to yield empty ghost cells with the bulk of the cell wall undisturbed. Northern blot analysis indicated that hol–lys genes under plac were co-transcribed, although the amount of hol transcript was larger than that of lys, ceasing via an apparently ρ-independent terminator just downstream of hol. However, deletion and/or fusion experiments suggested that: (1) the N-terminal half of Φg1e Hol composed of three putative transmembrane domains may be responsible for interaction with membrane; (2) the N-terminal end (five amino acids) seems nonessential; and (3) the C-terminal half containing charged amino acids appears to be involved in proper hol function. These results suggest that Φg1e Hol is a member of the lambdoid holin family, but divergent in several properties from lambda holin. | |||||
書誌情報 |
Gene -Amsterdam- 巻 197, 号 1-2, p. 137-145, 発行日 1997-09 |
|||||
出版者 | ||||||
出版者 | Elsevier Science B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 3781119 | |||||
書誌レコードID | ||||||
識別子タイプ | NCID | |||||
関連識別子 | TD00006847 | |||||
著者版フラグ | ||||||
出版タイプ | AO | |||||
出版タイプResource | http://purl.org/coar/version/c_b1a7d7d4d402bcce |