Evaluation of the impact of azo dyes on the metabolism of stabilized fecal communities and in vitro cell culture
Date
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The human gut microbiota is a complex and dynamic ecosystem of microbes existing in symbiosis with the host and can be altered by diet. Azo dyes are present in a large portion of our diet. To investigate the impact of azo dyes on gut microbial metabolites, a stabilized fecal slurry was subjected to Tartrazine exposure and metabolites were analyzed via 1-dimensional proton nuclear magnetic resonance. Results revealed that Tartrazine had a negative effect on 10 out of 13 profiled metabolites. Tartrazine had a negative impact on the transepithelial resistance of in vitro cultured Caco2 epithelial cells and increased the secretion of TNFα. Data from Guelph Health Family Studies suggested that children up to 6 years of age tend to consume 1.2 meals daily containing azo dye. This study suggests that dyes present in food interact with gut microbiota; the resulting metabolites may cause inflammation, leading to effects on human health.