In vitro toxicity of industrially relevant engineered nanoparticles in human alveolar epithelial cells: air–liquid interface versus submerged cultures
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Otros documentos de la autoría: Bessa, Maria João; Brandão, Fátima; Fokkens, Paul H. B.; Leseman, Daan L. A. C.; Boere, A. John F.; Cassee, Flemming R.; Salmatonidis, Apostolos; Viana, Mar; Vulpoi Lazar, Adriana; simon, Simion; Monfort, Eliseo; Paulo Teixeira, Joao; Fraga, Sónia
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7033
comunitat-uji-handle3:10234/8618
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INVESTIGACIONMetadatos
Título
In vitro toxicity of industrially relevant engineered nanoparticles in human alveolar epithelial cells: air–liquid interface versus submerged culturesAutoría
Fecha de publicación
2021-11-27Editor
MDPIISSN
2079-4991Cita bibliográfica
Bessa, M.J.; Brandão, F.; Fokkens, P.H.B.; Leseman, D.L.A.C.; Boere, A.J.F.; Cassee, F.R.; Salmatonidis, A.; Viana, M.; Vulpoi, A.; Simon, S.; et al. In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air–Liquid Interface versus Submerged Cultures. Nanomaterials 2021, 11Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Diverse industries have already incorporated within their production processes engineered nanoparticles (ENP), increasing the potential risk of worker inhalation exposure. In vitro models have been widely used to ... [+]
Diverse industries have already incorporated within their production processes engineered nanoparticles (ENP), increasing the potential risk of worker inhalation exposure. In vitro models have been widely used to investigate ENP toxicity. Air–liquid interface (ALI) cell cultures have been emerging as a valuable alternative to submerged cultures as they are more representative of the inhalation exposure to airborne nano-sized particles. We compared the in vitro toxicity of four ENP used as raw materials in the advanced ceramics sector in human alveolar epithelial-like cells cultured under submerged or ALI conditions. Submerged cultures were exposed to ENP liquid suspensions or to aerosolised ENP at ALI. Toxicity was assessed by determining LDH release, WST-1 metabolisation and DNA damage. Overall, cells were more sensitive to ENP cytotoxic effects when cultured and exposed under ALI. No significant cytotoxicity was observed after 24 h exposure to ENP liquid suspensions, although aerosolised ENP clearly affected cell viability and LDH release. In general, all ENP increased primary DNA damage regardless of the exposure mode, where an increase in DNA strand-breaks was only detected under submerged conditions. Our data show that at relevant occupational concentrations, the selected ENP exert mild toxicity to alveolar epithelial cells and exposure at ALI might be the most suitable choice when assessing ENP toxicity in respiratory models under realistic exposure conditions. [-]
Publicado en
Nanomaterials Vol. 11, Issue 12 (2021)Entidad financiadora
CERASAFE - ERA-NET SIINN | Portuguese Foundation for Science and Technology | European Regional Development Funds (FEDER/FNR) | Spanish Ministry of Science and Innovation | Romanian National Authority for Scientific Research and Innovation
Código del proyecto o subvención
FCT; SIINN/0004/2014 | PTDC/MED-TOX/31162/2017 | PCIN-2015-173-C02-01 / CEX2018-000794-S-Severo Ochoa | CCCDI-UEFISCDI 29/2016
Título del proyecto o subvención
NanoBioBarriers project
Derechos de acceso
info:eu-repo/semantics/openAccess
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