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Título: | Characterisation of 'starking' apple polyphenoloxidase |
Autor: | Rocha, Ada; Cano, M. Pilar CSIC ORCID ; Galeazzi, M.; Morais, Alcina | Palabras clave: | freezing Apple Lyophilisation Starking Storage Acetone powder |
Fecha de publicación: | 1998 | Editor: | John Wiley & Sons | Citación: | Journal of the Science of Food and Agriculture 77: 527- 534 (1998) | Resumen: | Experiments were performed to optimise the extraction conditions of 'Starking' apple fruit polyphenoloxidase (PPO), to evaluate the affinity and specificity towards several substrates and to study the stability of enzyme extracts from apple samples stored under different conditions. Sodium phosphate buffer (0.2 M, pH 6.5) plus 0.25% Triton X100 and 1% or 2% PVPP was found to be the most efficient extraction medium. Chlorogenic acid, dopamine and 4-methylcatechol showed similar specificity towards PPO, and chlorogenic acid was found to be the best substrate for the enzyme. Enzyme extracts from frozen cut apple stored at -4°C, and extracts from lyophilised apple samples stored at 4°C were more stable than extracts obtained from fresh-cut or acetone powder samples. | URI: | http://hdl.handle.net/10261/114868 | DOI: | 10.1002/(SICI)1097-0010(199808)77:4<527::AID-JSFA76>3.0.CO;2-E | Identificadores: | doi: 10.1002/(SICI)1097-0010(199808)77:4<527::AID-JSFA76>3.0.CO;2-E issn: 0022-5142 |
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