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Título: | A fast, reliable, ultra high performance liquid chromatography method for the simultaneous determination of amino acids, biogenic amines and ammonium ions in cheese, using diethyl ethoxymethylenemalonate as a derivatising agent |
Autor: | Redruello, Begoña CSIC ORCID; Ladero Losada, Víctor Manuel CSIC ORCID ; Cuesta Suárez, Isabel CSIC; Álvarez-Buylla, Jorge R. CSIC; Martín, M. Cruz CSIC ORCID ; Fernández García, María CSIC ORCID ; Álvarez González, Miguel Ángel CSIC ORCID | Palabras clave: | Cheeses Biogenic amines DEEMM UHPLC method Amino acid |
Fecha de publicación: | 15-ago-2013 | Editor: | Elsevier | Citación: | Food Chemistry 139(1-4): 1029-1035 (2013) | Resumen: | Derivatisation treatment with diethyl ethoxymethylenemalonate followed by ultra-HPLC allowed the simultaneous quantification of 22 amino acids, 7 biogenic amines and ammonium ions in cheese samples in under 10 min. This is the fastest elution time ever reported for such a resolution. The proposed method shows good linearity (R2 > 0.995) and sensitivity (detection limit 0.08–3.91 μM; quantification limit <13.02 μM). Intra- and inter-day repeatability ranged from 0.35% to 1.25% and from 0.85% to 5.2%, respectively. No significant effect of the cheese matrix was observed. | URI: | http://hdl.handle.net/10261/109548 | DOI: | 10.1016/j.foodchem.2013.01.071 | Identificadores: | doi: 10.1016/j.foodchem.2013.01.071 issn: 0308-8146 |
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