Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/112264
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Function of glutathione peroxidases in legume root nodules |
Autor: | Matamoros Galindo, Manuel Ángel CSIC ORCID ; Saiz Rodríguez, Ana CSIC; Peñuelas Hortelano, Maria CSIC ORCID; Bustos-Sanmamed, Pilar CSIC ORCID; Mulet-Salort, José Miguel CSIC ORCID; Barja, M. Victoria CSIC ORCID; Rouhier, Nicolas; Moore, Marten; James, Euan Kevin; Dietz, Karl-Josef; Becana Ausejo, Manuel CSIC ORCID | Palabras clave: | Antioxidants glutathione peroxidases legume nodules Lotus japonicus nitric oxide nitric oxide reactive oxygen species S-nitrosylation |
Fecha de publicación: | 2015 | Editor: | Oxford University Press | Citación: | Matamoros MA, Saiz A, Peñuelas M, Bustos-Sanmamed P, Mulet JM, Barja MV, Rouhier N, Moore M, James EK, Dietz KJ, Becana M. Function of glutathione peroxidases in legume root nodules. Journal of Experimental Botany 66 (10): 2979–2990 (2015) | Resumen: | Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far in legume nodules, despite the fact that reactive oxygen species are produced at different steps of the symbiosis. The function of two Gpxs that are highly expressed in nodules of the model legume Lotus japonicus was examined. Gene expression analysis, enzymatic and nitrosylation assays, yeast cell complementation, in situ mRNA hybridization, immunoelectron microscopy, and LjGpx-green fluorescent protein (GFP) fusions were used to characterize the enzymes and to localize each transcript and isoform in nodules. The LjGpx1 and LjGpx3 genes encode thioredoxin-dependent phospholipid hydroperoxidases and are differentially regulated in response to nitric oxide (NO) and hormones. LjGpx1 and LjGpx3 are nitrosylated in vitro or in plants treated with S-nitrosoglutathione (GSNO). Consistent with the modification of the peroxidatic cysteine of LjGpx3, in vitro assays demonstrated that this modification results in enzyme inhibition. The enzymes are highly expressed in the infected zone, but the LjGpx3 mRNA is also detected in the cortex and vascular bundles. LjGpx1 is localized to the plastids and nuclei, and LjGpx3 to the cytosol and endoplasmic reticulum. Based on yeast complementation experiments, both enzymes protect against oxidative stress, salt stress, and membrane damage. It is concluded that both LjGpxs perform major antioxidative functions in nodules, preventing lipid peroxidation and other oxidative processes at different subcellular sites of vascular and infected cells. The enzymes are probably involved in hormone and NO signalling, and may be regulated through nitrosylation of the peroxidatic cysteine essential for catalytic function. | Descripción: | 39 Pags.- 3 Tabls.- 10 Figs.- The definitive version is available at: http://jxb.oxfordjournals.org/ | Versión del editor: | http://dx.doi.org/10.1093/jxb/erv066 | URI: | http://hdl.handle.net/10261/112264 | DOI: | 10.1093/jxb/erv066 | ISSN: | 0022-0957 | E-ISSN: | 1460-2431 |
Aparece en las colecciones: | (EEAD) Artículos (IBMCP) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
BecanaM_JExpBot_2015.pdf | 5,11 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
19
checked on 11-mar-2024
SCOPUSTM
Citations
43
checked on 15-mar-2024
WEB OF SCIENCETM
Citations
36
checked on 25-feb-2024
Page view(s)
611
checked on 19-mar-2024
Download(s)
362
checked on 19-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.