Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/112493
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The GAGA factor of Drosophila interacts with SAP18, a Sin3-associated polypeptide |
Autor: | Espinàs, Maria Lluïsa CSIC ORCID ; Canudas, Silvia CSIC ORCID; Fanti, Laura; Pimpinelli, Sergio; Casanova, Jordi CSIC ORCID ; Azorín, Ferran CSIC ORCID | Fecha de publicación: | 2000 | Editor: | Nature Publishing Group | Citación: | EMBO Reports 1(3): 253-259 (2000) | Resumen: | SAP18, a polypeptide associated with the Sin3-HDAC co-repressor complex, was identified in a yeast two-hybrid screen as capable of interacting with the Drosophila GAGA factor. The interaction was confirmed in vitro by glutathione S-transferase pull-down assays using recombinant proteins and crude SL2 nuclear extracts. The first 245 residues of GAGA, including the POZ domain, are necessary and sufficient to bind dSAP18. In polytene chromosomes, dSAP18 and GAGA co-localize at a few discrete sites and, in particular, at the bithorax complex where GAGA binds some silenced polycomb response elements. When the dSAP18 dose is reduced, flies heterozygous for the GAGA mutation Tr/67show the homeotic transformation of segment A6 into A5, indicating that GAGA-dSAP18 interaction contributes to the functional regulation of the iab-6 element of the bithorax complex. These results suggest that, through recruitment of the Sin3-HDAC complex, GAGA might contribute to the regulation of homeotic gene expression. © 2000 European Molecular Biology Organization. | Versión del editor: | http://dx.doi.org/10.1093/embo-reports/kvd046 | URI: | http://hdl.handle.net/10261/112493 | DOI: | 10.1093/embo-reports/kvd046 | Identificadores: | doi: 10.1093/embo-reports/kvd046 issn: 1469-221X |
Aparece en las colecciones: | (IBMB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
19
checked on 21-abr-2024
SCOPUSTM
Citations
57
checked on 15-abr-2024
WEB OF SCIENCETM
Citations
55
checked on 29-feb-2024
Page view(s)
293
checked on 21-abr-2024
Download(s)
91
checked on 21-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.