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Título: | Transepithelial transport of dry-cured ham peptides with ACE inhibitory activity through a Caco-2 cell monolayer |
Autor: | Gallego, Marta CSIC ORCID; Grootaert, Charlotte; Mora, Leticia CSIC ORCID; Aristoy, María Concepción CSIC ORCID; van Camp, John; Toldrá Vilardell, Fidel CSIC ORCID | Palabras clave: | Dry-cured ham ACE inhibitory peptides Caco-2 cell monolayer Intestinal transport Mass spectrometry |
Fecha de publicación: | 6-ene-2016 | Editor: | Elsevier | Citación: | Journal of Functional Foods 21: 388-395 (2016) | Resumen: | Angiotensin converting enzyme (ACE) inhibitory peptides have been extensively studied as an alternative to synthetic drugs for the treatment of hypertension. Recent studies have shown that dry-cured ham is an important source of naturally generated bioactive peptides, especially showing ACE inhibitory activity. However, due to their excessive degradation by digestive and brush-border enzymes, it is not clear whether these peptides resist intestinal absorption and reach the blood stream where they may exert their antihypertensive effect. Therefore, dry-cured ham extracts and specific pure peptides naturally generated during the dry-curing process, showing ACE inhibitory activity, have been studied for their stability during transepithelial transport in a Caco-2 cell monolayer. The ACE inhibitory activity of transport samples was assayed, showing the highest values in apical samples at 15 min of incubation. In concentrated basal solutions, ACE inhibition values increased during transport for purified peptides, reaching values close to 70% for AAPLAP and KPVAAP at 60 min of cellular transport. Fragments generated by cellular activity were detected by using tandem mass spectrometry techniques, showing that AAATP, AAPLAP, and KPVAAP were hydrolysed during the transport, although KPVAAP was also absorbed intact. This study highlights the potential of intact dry-cured ham peptides as well as their fragments to be absorbed across the intestinal epithelium and reach the blood stream to exert an antihypertensive action. | Versión del editor: | http://dx.doi.org/10.1016/j.jff.2015.11.046 | URI: | http://hdl.handle.net/10261/137911 | DOI: | 10.1016/j.jff.2015.11.046 | ISSN: | 1756-4646 |
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J Func Foods 2016-388-395.pdf | Artículo principal + material suplementario | 1,77 MB | Adobe PDF | Visualizar/Abrir |
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