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Título: | Dissecting the Binding between Glutamine Synthetase and Its Two Natively Unfolded Protein Inhibitors |
Autor: | Pantoja-Uceda, D.; Neira, José L. CSIC ORCID; Saelices, Lorena CSIC ORCID; Robles-Rengel, Rocío CSIC ORCID; Florencio, Francisco J.; Muro-Pastor, M. Isabel CSIC ORCID ; Santoro, Jorge CSIC | Fecha de publicación: | 2016 | Editor: | American Chemical Society | Citación: | Biochemistry 55: 3370- 3382 (2016) | Resumen: | Ammonium is incorporated into carbon skeletons by the sequential action of glutamine synthetase (GS) and glutamate synthase (GOGAT) in cyanobacteria. The activity of Synechocystis sp. PCC 6803 GS type I is controlled by protein-protein interactions with two intrinsically disordered inactivating factors (IFs): the 65-residue (IF7) and the 149-residue one (IF17). In this work, we studied both IF7 and IF17 by nuclear magnetic resonance (NMR), and we described their binding to GS by using NMR and biolayer interferometry. We assigned the backbone nuclei of all residues of IF7. Analyses of chemical shifts and the N-{H} NOEs at two field strengths suggest that IF7 region Thr3-Arg13 and a few residues around Ser27 and Phe41 populated helical conformations (although the percentage is smaller around Phe41). The two-dimensional H-N HSQC and CON experiments suggest that IF17 populated several conformations. We followed the binding between GS and IF7 by NMR at physiological pH, and the residues interacting first with IF7 were Gln6 and Ser27, belonging to those regions that appeared to be ordered in the isolated protein. We also determined the k values and k values for the binding of both IF7 and IF17 to GS, where the GS protein was bound to a biosensor. The measurements of the kinetic constants for the binding of IF7 to GS suggest that: (i) binding does not follow a kinetic two-state model (GS+IF7 koffkon[IF7]GS:IF7), (ii) there is a strong electrostatic component in the determined k, and (iii) the binding is not diffusion-limited. | URI: | http://hdl.handle.net/10261/139752 | DOI: | 10.1021/acs.biochem.6b00072 | Identificadores: | doi: 10.1021/acs.biochem.6b00072 issn: 1520-4995 |
Aparece en las colecciones: | (IBVF) Artículos (IQF) Artículos |
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