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Título: | Tau regulates the localization and function of End-binding proteins 1 and 3 in developing neuronal cells |
Autor: | Sayas, Carmen Laura CSIC ORCID; Tortosa, Elena CSIC ORCID; Bollati, Flavia; Ramírez-Ríos, Sacnicte; Arnal, Isabelle; Ávila, Jesús CSIC ORCID | Palabras clave: | tau Neuronal development microtubule dynamics MAPs EBs +TIPs |
Fecha de publicación: | 2015 | Editor: | Blackwell Publishing | Citación: | Journal of Neurochemistry 133: 653- 667 (2015) | Resumen: | © 2015 International Society for Neurochemistry. The axonal microtubule-associated protein tau is a well-known regulator of microtubule stability in neurons. However, the putative interplay between tau and End-binding proteins 1 and 3 (EB1/3), the core microtubule plus-end tracking proteins, has not been elucidated yet. Here, we show that a cross-talk between tau and EB1/3 exists in developing neuronal cells. Tau and EBs partially colocalize at extending neurites of N1E- 115 neuroblastoma cells and axons of primary hippocampal neurons, as shown by confocal immunofluorescence analyses. Tau down-regulation leads to a reduction of EB1/3 comet length, as observed in shRNA-stably depleted neuroblastoma cells and TAU-/- neurons. EB1/3 localization depends on the expression levels and localization of tau protein. Overexpression of tau at high levels induces EBs relocalization to microtubule bundles at extending neurites of N1E-115 cells. In differentiating primary neurons, tau is required for the proper accumulation of EBs at stretches of microtubule bundles at the medial and distal regions of the axon. Tau interacts with EB proteins, as shown by immunoprecipitation in different nonneuronal and neuronal cells and in whole brain lysates. A tau/ EB1 direct interaction was corroborated by in vitro pull-down assays. Fluorescence recovery after photobleaching assays performed in neuroblastoma cells confirmed that tau modulates EB3 cellular mobility. In summary, we provide evidence of a new function of tau as a direct regulator of EB proteins in developing neuronal cells. This cross-talk between a classical microtubule-associated protein and a core microtubule plusend tracking protein may contribute to the fine-tuned regulation of microtubule dynamics and stability during neuronal differentiation. | URI: | http://hdl.handle.net/10261/140530 | DOI: | 10.1111/jnc.13091 | Identificadores: | doi: 10.1111/jnc.13091 issn: 1471-4159 |
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Avila J Tau regulates.pdf | 3,82 MB | Adobe PDF | Visualizar/Abrir |
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