Differences in chytridiomycosis infection costs between two amphibian species from Central Europe

Abstract

Batrachochytrium dendrobatidis (Bd) causes the disease chytridiomycosis associated with amphibian declines. Response and costs of infection varies greatly between species. Bd can induce a stress response in amphibians resulting in elevated corticosterone (CORT). We exposed Bombina variegata and Hyla arborea tadpoles to Bd+ or Bd-Salamandra salamandra larvae and measured CORT release rates, Bd infection loads, and survival through metamorphosis. Tadpoles of both species exposed to Bd+ larvae had elevated CORT release rates compared to tadpoles exposed to Bd-larvae. Bombina variegata appear less resistant to infection than H. arborea, showing higher Bd loads and more infected individuals. Within species, we did not find differences in cost of infection on survival, however more B. variegata tadpoles reached metamorphosis than H. arborea. The differences in resistance may be species specific, owing to higher immunity defenses with H. arborea having higher overall CORT release rates, and differences in antimicrobial peptides, or to differences in Bd strain or other unexplored mechanisms.