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Título: | Galactooligosaccharide Production from Pantoea anthophila Strains Isolated from “Tejuino”, a Mexican Traditional Fermented Beverage |
Autor: | Yáñez-Ñeco, Claudia; Rodríguez-Colinas, Bárbara CSIC; Amaya-Delgado, Lorena; Ballesteros Olmo, Antonio CSIC ORCID; Gschaedler, Anne; Plou Gasca, Francisco José CSIC ORCID ; Arrizón, Javier | Palabras clave: | Pantoea anthophila Permeabilized cells β-galactosidase Galactooligosaccharides Transglycosylation Prebiotics |
Fecha de publicación: | 22-ago-2017 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | Catalysts 7: 242 (2017) | Resumen: | Two Pantoea anthophila bacterial strains were isolated from “tejuino”, a traditional Mexican beverage, and studied as β-galactosidase producers for galactooligosaccharides synthesis. Using 400 g/L of lactose, 50 °C, and 15 U/mL of β-galactosidase activity with ethanol-permeabilized cells, the maximum galactooligosaccharides (GOS) yield determined by High performance anion exchange chromatography with pulse amperometric detection (HPAEC-PAD) was 136 g/L (34% w/w of total sugars) at 96% of lactose conversion for Bac 55.2 and 145 g/L (36% w/w of total sugars) at 94% of lactose conversion for Bac 69.1. The main synthesized products were the disaccharides allolactose [Gal-β(1 → 6)-Glc] and 6-galactobiose [Gal-β(1 → 6)-Gal], as well as the trisaccharides 3′-galactosyl-lactose [Gal-β(1 → 3)-Gal-β(1 → 4)-Glc], 6-galactotriose [Gal-β(1 → 6)-Gal-β(1 → 6)-Gal], 3′-galactosyl-allolactose [Gal-β(1 → 3)-Gal-β(1 → 6)-Glc], and 6′-galactosyl-lactose [Gal-β(1 → 6)-Gal-β(1 → 4)-Glc]. The β-galactosidases present in both strains showed a high transgalactosylation activity and formed principally β(1 → 3) and β(1 → 6) linkages. Considering the stability and bifidogenic properties of GOS containing such types of bonds, P. anthophila strains Bac 55.2 and Bac 69.1 possess a high potential as novel biocatalysts for prebiotic industrial production. | Versión del editor: | http://dx.doi.org/10.3390/catal7080242 | URI: | http://hdl.handle.net/10261/163802 | DOI: | 10.3390/catal7080242 | E-ISSN: | 2073-4344 |
Aparece en las colecciones: | (ICP) Artículos |
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