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Título: | A New Reversed Phase-HPLC Method Resolving All Major Higher Plant Photosynthetic Pigments |
Autor: | De Las Rivas, Javier CSIC ORCID CVN ; Abadía Bayona, Anunciación CSIC ORCID ; Abadía Bayona, Anunciación CSIC ORCID ; Abadía Bayona, Javier CSIC ORCID | Fecha de publicación: | sep-1989 | Editor: | American Society of Plant Biologists | Citación: | Plant Physiology 91(1): 190-192 (1989) | Resumen: | A new reversed phase-high performance liquid chromatography method has been developed to analyze the full complement of higher plant photosynthetic pigments (cis-neoxanthin, neoxanthin, violaxanthin, taraxanthin, anteraxanthin, lutein, zeaxanthin, cis-lutein, chlorophyll b, chlorophyll a, - and -carotene). The separation is carried out on a C18 column in about 10 minutes, using a single high-pressure pump and three different mobile phases in three isocratic steps. This method introduces a major improvement in higher plant photosynthetic pigment analysis, resolving in only 10 minutes all photosynthetic pigments while achieving good separation of lutein from its isomer zeaxanthin. Zeaxanthin is involved in the xanthophyll cycle, which recently has been proposed to play a significant role in the protection of the photosynthetic apparatus from photoinhibitory conditions (Demmig et al. [1987] Plant Physiol 84: 218-224). | Versión del editor: | http://www.plantphysiol.org/cgi/reprint/91/1/190 | URI: | http://hdl.handle.net/10261/16466 | DOI: | 10.1104/pp.91.1.190 | ISSN: | 0032-0889 1532-2548 |
Aparece en las colecciones: | (EEAD) Artículos |
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