Identification of constitutive metabolites in pea responsible for antixenosis and antibiosis against Bruchus pisorum

Abstract

Pea weevils (Bruchus pisorum) is a pest of the field pea (Pisum sativum) of great economic importance. Recently, pea accessions have been reported to reduce seed infestation, and/or larval development. Pods and seeds from these accessions were collected to check constitutive metabolites able to block the egg lying on pods (seed infestation) while pea seeds to check metabolites hampering larval development. Pea pods and seeds were lyophilized in order to evaluate metabolites that may be involved in pea weevil infestation. Standard method for metabolomics analysis was carried out on lipophilic and hydrophilic metabolites extracted, then compared to find a biomarker of resistance in pea. In this communication the studies aimed to identify metabolites responsible for antixenosis and/or antibiosis in pea resistant accessions to weevils will be discussed.