Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/174010
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Molecular characterization and antimicrobial resistance of STEC strains isolated from healthy cattle in 2011 and 2013 in Spain |
Autor: | Cabal, Adriana CSIC ORCID; Porrero, M. Concepción; Cruz, M. L. de la; Sáez, José L.; Bárcena, Carmen; Lopez, G.; Gortázar, Christian CSIC ORCID ; Domínguez, Lucas CSIC; Álvarez, Julio CSIC ORCID | Palabras clave: | Zoonotic foodborne diseases Molecular epidemiology Pulsed-field gel electrophoresis (PFGE) Public health Shiga-like toxin-producing E. coli |
Fecha de publicación: | 2016 | Editor: | Cambridge University Press | Citación: | Epidemiology and Infection 144(14): 2956-2966 (2016) | Resumen: | Prevention of Shiga toxin-producing Escherichia coli (STEC) foodborne outbreaks is hampered by its complex epidemiology. We assessed the distribution of virulence genes (VGs), main serogroups/serotypes for public health [haemolytic uraemic syndrome (HUS)-related], antimicrobial resistance (AMR) profiles and pulsed-field gel electrophoresis (PFGE) patterns in a collection of STEC isolates obtained from cattle hide (n = 149) and faecal (n = 406) samples collected during a national survey conducted in Spain in 2011 and 2013. Isolates were cultured using McConkey and CT-SMAC agar after enrichment, and confirmed as STEC by PCR. STEC prevalence in hides (15·4%) was higher than in faeces (10·7%) and O157:H7 was more frequent in the former (2·7% vs. 0·99%). Non-O157 HUS-related serogroups were present albeit at low frequencies. The non-O157 isolates were more heterogeneous than O157:H7 in their VG patterns, with 25/64 presenting VGs from both STEC and enterotoxigenic pathotypes (hybrid isolates). Of the STEC isolates, 62·5% were resistant at least to one antimicrobial, and no differences in AMR between O157:H7 and non-O157 were detected. All isolates had different profiles by PFGE and did not form a cluster. Overall, our results demonstrated that STEC in the cattle reservoir is still a matter of concern for human health due to the presence of HUS-related serogroups, the occurrence of certain VGs, AMR and the additional risks that hybrid isolates may pose, and thus warrants further investigation. | URI: | http://hdl.handle.net/10261/174010 | DOI: | 10.1017/S0950268816001370 | Identificadores: | doi: 10.1017/S0950268816001370 issn: 0950-2688 |
Aparece en las colecciones: | (IREC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
3
checked on 11-abr-2024
SCOPUSTM
Citations
10
checked on 24-abr-2024
WEB OF SCIENCETM
Citations
9
checked on 23-feb-2024
Page view(s)
209
checked on 23-abr-2024
Download(s)
84
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.