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Título

Crystallization and preliminary X-ray diffraction analysis of the beta subunit Yke2 of the Gim complex from Saccharomyces cerevisiae

AutorPérez de Diego, Rebeca; Ortiz-Lombardía, Miguel CSIC; Bravo, Jerónimo CSIC ORCID
Palabras clavePrefoldin
Beta-subunit
Gim complex
Yke2
X-ray diffraction
Fecha de publicación1-jun-2008
EditorInternational Union of Crystallography
CitaciónActa Crystallographica - Section F 64(Pt 6):501-3 (2008)
ResumenThe Gim complex (GimC) from Saccharomyces cerevisiae is a heterohexameric protein complex, also known as prefoldin (PFD), which binds and stabilizes unfolded target polypeptides and subsequently delivers them to chaperonins for completion of folding. In this study, the crystallization and preliminary X-ray analysis of one of the beta subunits of the Gim complex (Yke2) from S. cerevisiae are described. The purified protein was crystallized by the vapour-diffusion method, producing two types of crystals that belonged to the orthorhombic space group C222 or the primitive monoclinic space group P2(1). The unit-cell parameters for the C-centred orthorhombic crystal were a = 48.2, b = 168.86, c = 131.81 A and the unit-cell parameters for the primitive monoclinic crystal were a = 47.83, b = 134.90, c = 81.50 A, beta = 100.71 degrees . The Yke2 crystals diffracted to 4.2 and 3.1 A resolution, respectively, on a rotating-anode generator under cryoconditions. This is the first report concerning the crystallization of a beta subunit of a eukaryotic prefoldin.
Descripción3 páginas, 2 figuras, 1 tabla
Versión del editorhttp://dx.doi.org/10.1107/S1744309108011846
URIhttp://hdl.handle.net/10261/176457
DOI10.1107/S1744309108011846
E-ISSN1744-3091
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