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Título: | Crystallization and preliminary X-ray diffraction analysis of the beta subunit Yke2 of the Gim complex from Saccharomyces cerevisiae |
Autor: | Pérez de Diego, Rebeca; Ortiz-Lombardía, Miguel CSIC; Bravo, Jerónimo CSIC ORCID | Palabras clave: | Prefoldin Beta-subunit Gim complex Yke2 X-ray diffraction |
Fecha de publicación: | 1-jun-2008 | Editor: | International Union of Crystallography | Citación: | Acta Crystallographica - Section F 64(Pt 6):501-3 (2008) | Resumen: | The Gim complex (GimC) from Saccharomyces cerevisiae is a heterohexameric protein complex, also known as prefoldin (PFD), which binds and stabilizes unfolded target polypeptides and subsequently delivers them to chaperonins for completion of folding. In this study, the crystallization and preliminary X-ray analysis of one of the beta subunits of the Gim complex (Yke2) from S. cerevisiae are described. The purified protein was crystallized by the vapour-diffusion method, producing two types of crystals that belonged to the orthorhombic space group C222 or the primitive monoclinic space group P2(1). The unit-cell parameters for the C-centred orthorhombic crystal were a = 48.2, b = 168.86, c = 131.81 A and the unit-cell parameters for the primitive monoclinic crystal were a = 47.83, b = 134.90, c = 81.50 A, beta = 100.71 degrees . The Yke2 crystals diffracted to 4.2 and 3.1 A resolution, respectively, on a rotating-anode generator under cryoconditions. This is the first report concerning the crystallization of a beta subunit of a eukaryotic prefoldin. | Descripción: | 3 páginas, 2 figuras, 1 tabla | Versión del editor: | http://dx.doi.org/10.1107/S1744309108011846 | URI: | http://hdl.handle.net/10261/176457 | DOI: | 10.1107/S1744309108011846 | E-ISSN: | 1744-3091 |
Aparece en las colecciones: | (IBV) Artículos |
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2008 Acta Crystallogr Sect F Struct Biol Cryst Commun 64-501 vers aut.pdf | 353,7 kB | Adobe PDF | Visualizar/Abrir |
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