Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/203042
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Cytosine-5 RNA methylation links protein synthesis to cell metabolism |
Autor: | Gkatza, Nikoletta A.; Castro, Cecilia; Harvey, Robert F.; Heiß, Matthias; Popis, Martyna C.; Blanco, Sandra CSIC ORCID CVN | Fecha de publicación: | 14-jun-2019 | Editor: | Public Library of Science | Citación: | PLoS Biology 17(6): e3000297 (2019) | Resumen: | Posttranscriptional modifications in transfer RNA (tRNA) are often critical for normal development because they adapt protein synthesis rates to a dynamically changing microenvironment. However, the precise cellular mechanisms linking the extrinsic stimulus to the intrinsic RNA modification pathways remain largely unclear. Here, we identified the cytosine-5 RNA methyltransferase NSUN2 as a sensor for external stress stimuli. Exposure to oxidative stress efficiently repressed NSUN2, causing a reduction of methylation at specific tRNA sites. Using metabolic profiling, we showed that loss of tRNA methylation captured cells in a distinct catabolic state. Mechanistically, loss of NSUN2 altered the biogenesis of tRNA-derived noncoding fragments (tRFs) in response to stress, leading to impaired regulation of protein synthesis. The intracellular accumulation of a specific subset of tRFs correlated with the dynamic repression of global protein synthesis. Finally, NSUN2-driven RNA methylation was functionally required to adapt cell cycle progression to the early stress response. In summary, we revealed that changes in tRNA methylation profiles were sufficient to specify cellular metabolic states and efficiently adapt protein synthesis rates to cell stress. | Descripción: | © The Author(s). | Versión del editor: | http://dx.doi.org/10.1371/journal.pbio.3000297 | URI: | http://hdl.handle.net/10261/203042 | DOI: | 10.1371/journal.pbio.3000297 | ISSN: | 1544-9173 | E-ISSN: | 1545-7885 |
Aparece en las colecciones: | (IBMCC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Cytosine-5_Gkatza_Art2019.pdf | 4,24 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
56
checked on 12-abr-2024
SCOPUSTM
Citations
76
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
76
checked on 29-feb-2024
Page view(s)
153
checked on 19-abr-2024
Download(s)
143
checked on 19-abr-2024