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| Título: | H3K4me2 accompanies chromatin immaturity in human spermatozoa: an epigenetic marker for sperm quality assessment |
Autor: | Štiavnická, Miriama; García-Álvarez, Olga CSIC ORCID; Nevoral, Jan | Palabras clave: | Sperm DNA H3K4me2 Epigenetics Chromatin immaturity High DNA stainability |
Fecha de publicación: | 2020 | Editor: | Taylor & Francis | Citación: | Systems Biology in Reproductive Medicine 66(1): 3-11 (2020) | Resumen: | Chromatin remodeling, including histone post-translational modifications, during spermatogenesis can affect sperm quality and fertility, and epigenetic marks may therefore be useful for clinical evaluations of sperm. Together with histone hyperacetylation, the dimethylation of histone H3 on lysine K4 (H3K4me2) is also required during protamination. Accordingly, we evaluated the utilization of this epigenetic mark for the identification of sperm with decrease quality and immature chromatin. In this study, 99 semen samples, including 22 normozoospermic (N), 63 asthenozoospermic (A), and 14 oligoasthenozoospermic (OA) samples, were comprehensively analyzed with respect to H3K4me2 levels, DNA damage (DNA fragmentation index, DFI), and sperm immaturity (high DNA stainability, %HDS), as determined by a sperm chromatin structure assay using flow cytometry. We detected a significant relationship between H3K4me2 and %HDS (r = 0.47; p < 0.001). Furthermore, we observed negative correlations between H3K4me2 and sperm concentration, motility, and mitochondrial activity (p < 0.05). The increase in immaturity as semen quality decreased (N > A > OA) indicates the importance of chromatin immaturity and histone code deviations in sperm evaluations. Using various approaches, our study elucidated H3K4me2 as a molecular marker of sperm quality with potential use in reproductive medicine. | Descripción: | Research Communication. et al. | Versión del editor: | https://doi.org/10.1080/19396368.2019.1666435 | URI: | http://hdl.handle.net/10261/215597 | DOI: | 10.1080/19396368.2019.1666435 | ISSN: | 1939-6368 | E-ISSN: | 1939-6376 |
| Aparece en las colecciones: | (IREC) Artículos |
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