Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/229250
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Improvements on marker-free images alignment for electron tomography

AutorSorzano, Carlos Óscar S.; Isidro, Federico de; Fernández-Giménez, Elena; Herreros, David ; Marco, S.; Carazo, José M.; Messaoudi, C.
Palabras claveElectron tomography
Tilt series alignment
Image processing
Fecha de publicación2020
EditorElsevier
CitaciónJournal of Structural Biology: X: 4: 100037 (2020)
ResumenElectron tomography is a technique to obtain three-dimensional structural information of samples. However, the technique is limited by shifts occurring during acquisition that need to be corrected before the reconstruction process. In 2009, we proposed an approach for post-acquisition alignment of tilt series images. This approach was marker-free, based on patch tracking and integrated in free software. Here, we present improvements to the method to make it more reliable, stable and accurate. In addition, we modified the image formation model underlying the alignment procedure to include different deformations occurring during acquisition. We propose a new way to correct these computed deformations to obtain reconstructions with reduced artifacts. The new approach has demonstrated to improve the quality of the final 3D reconstruction, giving access to better defined structures for different transmission electron tomography methods: resin embedded STEM-tomography and cryo-TEM tomography. The method is freely available in TomoJ software.
Descripción© 2020 The Author(s).
Versión del editorhttp://dx.doi.org/10.1016/j.yjsbx.2020.100037
URIhttp://hdl.handle.net/10261/229250
DOI10.1016/j.yjsbx.2020.100037
ISSN2590-1524
Aparece en las colecciones: (CNB) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Improvements_Sorzano_PV_Art2020.pdf10,09 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

4
checked on 28-mar-2024

SCOPUSTM   
Citations

9
checked on 27-mar-2024

WEB OF SCIENCETM
Citations

8
checked on 25-feb-2024

Page view(s)

80
checked on 28-mar-2024

Download(s)

79
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


Este item está licenciado bajo una Licencia Creative Commons Creative Commons