Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24146
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

The Complex Spatio-Temporal Regulation of the Drosophila Myoblast Attractant Gene duf/kirre

AutorGuruharsha, K.G.; Ruiz-Gómez, Mar CSIC ORCID; Ranganath, H. A.; Siddharthan, Rahul; VijayRaghavan, K.
Palabras claveDrosophila
duf/kirre
Fecha de publicación9-sep-2009
EditorPublic Library of Science
CitaciónPLoS ONE 4(9): e6960
ResumenA key early player in the regulation of myoblast fusion is the gene dumbfounded (duf, also known as kirre). Duf must be expressed, and function, in founder cells (FCs). A fixed number of FCs are chosen from a pool of equivalent myoblasts and serve to attract fusion-competent myoblasts (FCMs) to fuse with them to form a multinucleate muscle-fibre. The spatial and temporal regulation of duf expression and function are important and play a deciding role in choice of fibre number, location and perhaps size. We have used a combination of bioinformatics and functional enhancer deletion approaches to understand the regulation of duf. By transgenic enhancer-reporter deletion analysis of the duf regulatory region, we found that several distinct enhancer modules regulate duf expression in specific muscle founders of the embryo and the adult. In addition to existing bioinformatics tools, we used a new program for analysis of regulatory sequence, PhyloGibbs-MP, whose development was largely motivated by the requirements of this work. The results complement our deletion analysis by identifying transcription factors whose predicted binding regions match with our deletion constructs. Experimental evidence for the relevance of some of these TF binding sites comes from available ChIP-on-chip from the literature, and from our analysis of localization of myogenic transcription factors with duf enhancer reporter gene expression. Our results demonstrate the complex regulation in each founder cell of a gene that is expressed in all founder cells. They provide evidence for transcriptional control—both activation and repression—as an important player in the regulation of myoblast fusion. The set of enhancer constructs generated will be valuable in identifying novel trans-acting factor-binding sites and chromatin regulation during myoblast fusion in Drosophila. Our results and the bioinformatics tools developed provide a basis for the study of the transcriptional regulation of other complex genes.
Versión del editorhttp://dx.doi.org/10.1371/journal.pone.0006960
URIhttp://hdl.handle.net/10261/24146
DOI10.1371/journal.pone.0006960
ISSN1932-6203
Aparece en las colecciones: (CBM) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
KGGuruharsha_Plos One_e6960.pdf2,42 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

4
checked on 29-feb-2024

SCOPUSTM   
Citations

9
checked on 28-mar-2024

WEB OF SCIENCETM
Citations

9
checked on 28-feb-2024

Page view(s)

347
checked on 28-mar-2024

Download(s)

253
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.