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Título: | Transcriptional repression by Pax5 (BSAP) through interaction with corepressors of the Groucho family |
Autor: | Eberhard, Eberhard; Jiménez, Gerardo CSIC ORCID ; Jiménez, Gerardo CSIC ORCID ; Heavey, Barry; Busslinger, Meinrad | Palabras clave: | Groucho (Grg) Interaction partners Pax5 (BSAP) Transcriptional repression |
Fecha de publicación: | 15-may-2000 | Editor: | Nature Publishing Group | Citación: | EMBO Journal 19(10): 2292–2303 (2000) | Resumen: | Pax5 (BSAP) functions as both a transcriptional activator and repressor during midbrain patterning, B-cell development and lymphomagenesis. Here we demonstrate that Pax5 exerts its repression function by recruiting members of the Groucho corepressor family. In a yeast two-hybrid screen, the groucho-related gene product Grg4 was identified as a Pax5 partner protein. Both proteins interact cooperatively via two separate domains: the N-terminal Q and central SP regions of Grg4, and the octapeptide motif and C-terminal transactivation domain of Pax5. The phosphorylation state of Grg4 is altered in vivo upon Pax5 binding. Moreover, Grg4 efficiently represses the transcriptional activity of Pax5 in an octapeptide-dependent manner. Similar protein interactions resulting in transcriptional repression were also observed between distantly related members of both the Pax2/5/8 and Groucho protein families. In agreement with this evolutionary conservation, the octapeptide motif of Pax proteins functions as a Groucho-dependent repression domain in Drosophila embryos. These data indicate that Pax proteins can be converted from transcriptional activators to repressors through interaction with corepressors of the Groucho protein family. | Descripción: | 12 pages, 10 figures.-- PMID: 10811620 [PubMed].-- PMCID: PMC384353. | Versión del editor: | http://dx.doi.org/10.1093/emboj/19.10.2292 | URI: | http://hdl.handle.net/10261/26320 | DOI: | 10.1093/emboj/19.10.2292 | ISSN: | 0261-4189 | E-ISSN: | 1460-2075 |
Aparece en las colecciones: | (IQAC) Artículos |
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