Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/26765
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Biotransformations of putative phytoecdysteroid biosynthetic precursors in tissue cultures of Polypodium vulgare |
Autor: | Reixach, Natàlia; Lafont, René; Camps Díez, Francisco CSIC; Casas, Josefina CSIC ORCID | Palabras clave: | 20-Hydroxyecdysone Fern Hydroxycholesterol derivatives In vitro cultures Induction |
Fecha de publicación: | dic-1999 | Editor: | Federation of European Biochemical Societies | Citación: | FEBS Journal 266(2): 608-615 (1999) | Resumen: | Incubation of calli and prothalli of Polypodium vulgare with different tritium-labelled ecdysteroids has led to modification of some previous assumptions about the biosynthesis of ecdysteroids in plants. Thus, 25-deoxy-20-hydroxyecdysone was transformed efficiently in both tissues into 20-hydroxyecdysone (20E), but no 25-deoxyecdysteroids such as pterosterone and inokosterone were formed. Likewise, incubation of 2-deoxyecdysone (2dE) produced exclusively ecdysone (E) and 20E, indicating a high 2-hydroxylase activity in both tissues, despite calli not producing phytoecdysteroids. This 2-hydroxylation was also evident in the transformation of 2,22-dideoxyecdysone (2,22dE) into 22-deoxyecdysone (22dE). Different ecdysteroids that do not occur in P. vulgare were formed in the incubation of 3-dehydro-2,22,25-trideoxyecdysone (3D2,22,25dE) by 3α-reduction and 3β-reduction and 25-hydroxylation processes. The fact that 22,25-dideoxyecdysone and 22dE were the only 2-hydroxylated products formed in this case suggests that only compounds bearing a 3β-hydroxyl group are substrates for the 2-hydroxylase. Surprisingly, 22-hydroxylation was never observed with either 2,22dE or 3D2,22,25dE, raising the possibility that it could occur at an early step in the biosynthetic pathway. In this respect, labelled 22R-hydroxycholesterol was efficiently converted into E and 20E, whereas 22S-hydroxycholesterol was not transformed into ecdysteroids, because of its unsuitable configuration at C22. Finally, the conversion of 25-hydroxycholesterol into E and 20E was greatly enhanced after thermal treatment of prothalli which induces the release of previously stored ecdysteroids. Thus, P. vulgare prothalli and calli appear to be particularly suitable models for the study of ecdysteroid biosynthesis and its regulation in plants. | Descripción: | 8 pages, 7 figures.-- PMID: 10561604 [PubMed].-- Available online Dec 25, 2001. | Versión del editor: | http://dx.doi.org/10.1046/j.1432-1327.1999.00905.x | URI: | http://hdl.handle.net/10261/26765 | DOI: | 10.1046/j.1432-1327.1999.00905.x | ISSN: | 0014-2956 | E-ISSN: | 1432-1033 |
Aparece en las colecciones: | (IQAC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Reixach_Natalia_et_al.pdf | 268,31 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
13
checked on 22-abr-2024
WEB OF SCIENCETM
Citations
10
checked on 28-feb-2024
Page view(s)
397
checked on 24-abr-2024
Download(s)
315
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.