Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/2971
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | In vivo antioxidant treatment protects against bleomycin-induced lung damage in rats |
Autor: | Serrano-Mollar, Anna CSIC ORCID; Closa, Daniel CSIC ORCID; Morcillo, Esteban J.; Bulbena, Oriol CSIC | Palabras clave: | Pulmonary fibrosis Bleomycin Rat N-acetylcysteine Inflammation |
Fecha de publicación: | 26-mar-2003 | Editor: | Wiley-Blackwell | Citación: | British Journal of Pharmacology 138(6): 1037–1048 (2003) | Resumen: | This study examines the activity of the antioxidant N-acetylcysteine on bleomycin-induced pulmonary fibrosis in rats with emphasis on the early inflammatory phase. Rats receiving N-acetylcysteine (300 mg kg−1 day−1, intraperitoneal) had less augmented lung wet weight, and lower levels of proteins, lactate dehydrogenase, neutrophil and macrophage counts in bronchoalveolar lavage fluid and lung myeloperoxidase activity with a betterment of histological score at 3 days postbleomycin. A diminished lung GSH/GSSG ratio and augmented lipid hydroperoxides were observed 3 days postbleomycin. These changes were attenuated by N-acetylcysteine. Alveolar macrophages from bleomycin-exposed rats released augmented amounts of superoxide anion and nitric oxide. N-Acetylcysteine did not modify superoxide anion generation but reduced the increased production of nitric oxide. N-Acetylcysteine suppressed the bleomycin-induced increased activation of lung NF-κB (shift assay and immunohistochemistry), and decreased the augmented levels of the early inflammatory cytokines, tumour necrosis factor-α, interleukin-β, interleukin-6 and macrophage inflammatory protein-2 observed in bronchoalveolar lavage fluid at 1 and 3 days postbleomycin exposure. At 15 days postbleomycin, N-acetylcysteine decreased collagen deposition in bleomycin-exposed rats (hydroxyproline content: 6351±669 and 4626±288 μg per lung in drug vehicle- and N-acetylcysteine-treated rats, respectively; P<0.05). Semiquantitative histological assessment at this stage showed less collagen deposition in N-acetylcysteine-treated rats compared to those receiving bleomycin alone. These results indicate that N-acetylcysteine reduces the primary inflammatory events, thus preventing cellular damage and the subsequent development of pulmonary fibrosis in the bleomycin rat model. | Versión del editor: | http://dx.doi.org/10.1038/sj.bjp.0705138 | URI: | http://hdl.handle.net/10261/2971 | DOI: | 10.1038/sj.bjp.0705138 | ISSN: | 0007-1188 |
Aparece en las colecciones: | (IIBB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
In vivo antioxidant.pdf | 467,57 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
38
checked on 10-feb-2024
SCOPUSTM
Citations
116
checked on 13-mar-2024
WEB OF SCIENCETM
Citations
104
checked on 26-feb-2024
Page view(s)
400
checked on 18-mar-2024
Download(s)
426
checked on 18-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.