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Título: | Initial characterization of a potential transcriptional enhancer for the human c-K-ras gene |
Autor: | Jordano, Juan CSIC ORCID ; Perucho, M. | Palabras clave: | Beta globin Cell culture Genetic transcription Hela cell; Human Human cells Promoter region Base sequence DNA-binding proteins Globins Enhancer elements (genetics) |
Fecha de publicación: | 1988 | Editor: | Nature Publishing Group | Citación: | Oncogene 2 (4): 359-366 (1988) | Resumen: | The nucleotide sequence of the 5' end distal region of the human c-K-ras gene promoter was determined. This region, coincident with a variable DNAase I hypersensitive site in native chromatin, contains sequence similarities with known enhancers. A 400bp MstII DNA fragment of this region stimulated in cis the correctly initiated transcription of the human β-globin gene in transfected Hela cells. The stimulation of β-globin transcription (5-6 fold) was dependent on the distance and orientation of the c-K-ras sequences and on the presence of the CCAAT and CACCC elements in the β-globin promoter. Interaction of nuclear factors with these c-K-ras sequences was analysed by DNAase I footprinting assays using Hela nuclear extracts. A protein binding to these sequences was identified as nuclear factor 1 (NF-1) by DNAase I competition footprinting experiments. However, disruption of the c-K-ras NF-1 binding site by insertion mutagenesis had no effect on the transcriptional activity of the c-K-ras element. | Descripción: | Molecular Sequence Numbers GENBANK, X07536(submitted), Y00666(submitted) | Versión del editor: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0023895399&partnerID=40&md5=4b323eecb4972e838374c2df2e624604 | URI: | http://hdl.handle.net/10261/32583 | ISSN: | 0950-9232 | E-ISSN: | 1476-5594 |
Aparece en las colecciones: | (IRNAS) Artículos |
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