Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/43037
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Spectroscopic Study of the CP43′ Complex and the PSI–CP43′ Supercomplex of the Cyanobacterium Synechocystis PCC 6803

AutorFeng, Ximao; Neupane, Bhanu; Acharya, Khem; Zazubovich, Valter; Picorel Castaño, Rafael CSIC ORCID ; Seibert, Michael; Jankowiak, Ryszard J.
Palabras claveHole Burning
Fluorescence Excitation
Excitation Energy Transfer
Fecha de publicaciónnov-2011
EditorAmerican Chemical Society
CitaciónFeng X, Neupane B, Acharya K, Zazubovich V, Picorel RE, Seibert M, Jankowiak R. Spectroscopic Study of the CP43′ Complex and the PSI–CP43′ Supercomplex of the Cyanobacterium Synechocystis PCC 6803. Journal of Physical Chemistry B 115 (45): 13339–13349 (2011)
ResumenThe PSI-CP43' supercomplex of the cyanobacterium Synechocystis PCC 6803, grown under iron-starvation conditions, consists of a trimeric core Photosystem I (PSI) complex and an outer ring of 18 CP43' light-harvesting complexes. We have investigated the electronic structure and excitation energy transfer (EET) pathways within the CP43' (also known as the isiA gene product) ring using low-temperature absorption, fluorescence, fluorescence excitation, and hole-burning (HB) spectroscopies. Analysis of the absorption spectra of PSI, CP43', and PSI-CP43' complexes suggests that there are 13 chlorophylls (Chls) per CP43' monomer, i.e., a number that was observed in the CP43 complex of Photosystem 11 (PSII) (Umena, Y. et al. Nature 2011, 473, 55-60). This is in contrast with the recent modeling studies of Zhang et al. (Biochim. Biophys. Acta 2010, 1797, 457-465), which suggested that IsiA likely contains 15 Chls. Modeling studies of various optical spectra of the CP43' ring using the uncorrelated EET model (Zazubovich, V.; Jankowiak, R J. Lumin. 2007, 127, 245-250) suggest that CP43' monomers (in analogy to the CP43 complexes of the PSII core) also possess two quasi-degenerate low-energy states, A' and B'. The site distribution functions of states A' and B' maxima/full width at half-maximum (fwhm) are at 684 nm/180 cm(-1) and 683 nm/80 cm(-1), respectively. Our analysis shows that pigments mostly contributing to the lowest-energy A' and B' states must be located on the side of the CP43' complex facing the PSI core, a finding that contradicts the model of Zhang et al. but is in agreement with the model suggested by Nield et al. (Biochemistry 2003, 42, 3180-3188). We demonstrate that the A'-A' and B'-B' EET between different monomers is possible, though with a slower rate than intramonomer A'-B' and/or B'-A' energy transfer.
Descripción8 Pags. The definitive version, with Figs., is available at: http://pubs.acs.org/journal/jpcbfk
Versión del editorhttp://dx.doi.org/10.1021/jp206054b
URIhttp://hdl.handle.net/10261/43037
DOI10.1021/jp206054b
ISSN1520-6106
E-ISSN1520-5207
Aparece en las colecciones: (EEAD) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
PicorelR_JPhysChemB_17-11-2011.pdf97,53 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

29
checked on 12-mar-2024

WEB OF SCIENCETM
Citations

29
checked on 13-feb-2024

Page view(s)

432
checked on 18-mar-2024

Download(s)

334
checked on 18-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.