Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/43048
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Targeted gene expression profiling in European sea bass (Dicentrarchus labrax, L.) follicles from primary growth to late vitellogenesis |
Autor: | García-López, Ángel CSIC ORCID; Sánchez-Amaya, María Isabel CSIC; Prat, Francisco CSIC ORCID | Palabras clave: | Teleost fish Ovary Follicle Folliculogenesis Oonenesis Gene expression |
Fecha de publicación: | 12-jul-2011 | Editor: | Elsevier | Citación: | Comparative Biochemistry and Physiology - A - Molecular and Integrative Physiology 160(3): 374-380 (2011) | Resumen: | A real-time PCR-based gene expression survey was performed on isolated European sea bass follicles from primary growth to late vitellogenesis. Expression levels of 18 transcripts with demonstrated relevance during oogenesis, encoding gonadotropin, thyrotropin, estrogen, androgen, and vitellogenin receptors, steroidogenesis-related as well as growth and transcription factors were measured. Primary oocytes showed high mRNA levels of insulin-like growth factors 1 and 2, bone morphogenetic protein 4, estrogen receptor 2b, androgen receptor b, and SRY-box containing gene 17 together with low transcript amounts of gonadotropin receptors. Follicles at the lipid vesicles stage (i.e., the beginning of the secondary growth phase) showed elevated mRNA amounts of follicle stimulating hormone receptor (fshr) and anti-Mullerian hormone. Early-to-mid vitellogenic follicles showed high mRNA levels of fshr and cytochrome P450, family 19, subfamily A, polypeptide 1a while mid-to-late vitellogenic follicles expressed increasing transcript amounts of luteinizing hormone/choriogonadotropin receptor, steroidogenic acute regulatory protein, and estrogen receptors 1 and 2a. The molecular data presented here may serve as a solid base for future studies focused on unraveling the specific mechanisms orchestrating follicular development in teleost fish. | Descripción: | 7 páginas, 6 figuras, 1 tabla como material suplementario. | Versión del editor: | http://dx.doi.org/10.1016/j.cbpa.2011.07.006 | URI: | http://hdl.handle.net/10261/43048 | DOI: | 10.1016/j.cbpa.2011.07.006 | ISSN: | 1095-6433 | E-ISSN: | 1531-4332 |
Aparece en las colecciones: | (ICMAN) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
european_sea_bass_Garcia.pdf | 429,39 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
38
checked on 18-abr-2024
WEB OF SCIENCETM
Citations
36
checked on 29-feb-2024
Page view(s)
344
checked on 24-abr-2024
Download(s)
329
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.