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Título: | The use of green fluorescent protein as a marker for Brucella vaccines |
Autor: | Chacón Diaz, Carlos; Muñoz-Rodríguez, Melissa; Barquero-Calvo, Elías; Guzmán Verri, Caterina; Chaves Olarte, Esteban; Grilló, María Jesús CSIC ORCID ; Moreno Robles, Eduardo | Palabras clave: | Brucella Brucellosis Vaccines S19 Rev1 GFP Diagnostic tests ELISA |
Fecha de publicación: | 10-ene-2011 | Editor: | Elsevier | Citación: | Vaccine 29(3): 577-582 (2011) | Resumen: | Brucellosis is an important malady of productive and wildlife animals and a worldwide zoonosis. The use of effective vaccines and the corresponding diagnostic tests that allow differentiating infected from vaccinated animals are essential tools to control the disease. For this, a prototype of Brucella abortus S19 vaccine expressing green fluorescent protein (S19-GFP) was constructed. The S19-GFP was readily identified under ultraviolet light by macroscopic and microscopic examination and maintained all the biochemical characteristics of the parental S19 vaccine. S19-GFP replicated ex vivo and in vivo, and protected mice against challenge with virulent B. abortus to the same extent as the isogenic S19. An immunoenzymatic assay designed to measure anti-GFP antibodies allowed the discrimination between mice vaccinated with S19-GFP and those immunized with S19. Both vaccines raised antibodies against lipopolysaccharide molecule to similar levels. This experimental model constitutes a “proof of concept” for the use of Brucella-GFP vaccines and associated diagnostic tests to distinguish vaccinated from naturally Brucella infected animals. | Descripción: | 6 p., 3 figures, 1 table and references | Versión del editor: | http://dx.doi.org/10.1016/j.vaccine.2010.09.109 | URI: | http://hdl.handle.net/10261/49692 | DOI: | 10.1016/j.vaccine.2010.09.109 | ISSN: | 0264-410X | E-ISSN: | 1873-2518 |
Aparece en las colecciones: | (IDAB) Artículos |
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Fichero | Descripción | Tamaño | Formato | |
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Artículo S19-GFP Revisado_1-08-2010_MJG.pdf | Artículo principal | 130,61 kB | Adobe PDF | Visualizar/Abrir |
Figuras paper S19-GFP 1-08-10.pdf | Figuras | 135,02 kB | Adobe PDF | Visualizar/Abrir |
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