Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/52502
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Microtubule nucleation at the cis-side of the golgi apparatus requires AKAP450 and GM130 |
Autor: | Rivero, Sabrina CSIC ORCID; Cárdenas, Jesús CSIC; Bornens, Michel; Ríos, Rosa M. CSIC ORCID | Palabras clave: | AKAP450 Centrosomes GM130 Golgi apparatus MTs |
Fecha de publicación: | 22-abr-2009 | Editor: | Nature Publishing Group | Citación: | EMBO Journal 28(8): 1016-1028 (2009) | Resumen: | We report that microtubule (MT) nucleation at the Golgi apparatus requires AKAP450, a centrosomal -TuRC-interacting protein that also forms a distinct network associated with the Golgi. Depletion of AKAP450 abolished MT nucleation at the Golgi, whereas depletion of the cis-Golgi protein GM130 led to the disorganisation of AKAP450 network and impairment of MT nucleation. Brefeldin-A treatment induced relocalisation of AKAP450 to ER exit sites and concomitant redistribution of MT nucleation capacity to the ER. AKAP450 specifically binds the cis-side of the Golgi in an MT-independent, GM130-dependent manner. Short AKAP450-dependent growing MTs are covered by CLASP2. Like for centrosome, dynein/dynactin complexes are necessary to anchor MTs growing from the Golgi. We further show that Golgi-associated AKAP450 has a role in cell migration rather than in cell polarisation of the centrosome-Golgi apparatus. We propose that the recruitment of AKAP450 on the Golgi membranes through GM130 allows centrosome-associated nucleating activity to extend to the Golgi, to control the assembly of subsets of MTs ensuring specific functions within the Golgi or for transporting specific cargos to the cell periphery.© 2009 European Molecular Biology Organization / All Rights Reserved. | URI: | http://hdl.handle.net/10261/52502 | DOI: | 10.1038/emboj.2009.47 | Identificadores: | doi: 10.1038/emboj.2009.47 issn: 0261-4189 |
Aparece en las colecciones: | (CABIMER) Artículos |
Mostrar el registro completo
CORE Recommender
PubMed Central
Citations
155
checked on 13-abr-2024
SCOPUSTM
Citations
254
checked on 15-abr-2024
WEB OF SCIENCETM
Citations
245
checked on 27-feb-2024
Page view(s)
330
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.