Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/61288
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Mesenchymal stem cells from multiple myeloma patients display distinct genomic profile as compared with those from normal donorsGenomic profile of multiple myeloma MSCs |
Autor: | Garayoa, Mercedes CSIC ORCID ; García, Juan L. CSIC ORCID CVN ; Santamaría, Carlos; García-Gómez, Antonio; Blanco, Juan F.; Pandiella, Atanasio CSIC ORCID CVN ; Hernández, Jesús M. CSIC ORCID ; Sánchez-Guijo, Fermín M. CSIC ORCID; Cañizo, María Consuelo del; Gutiérrez, Norma Carmen; San Miguel, Jesús F. CSIC ORCID | Fecha de publicación: | 2009 | Editor: | Nature Publishing Group | Citación: | Leukemia 23: 1515-1527 (2009) | Resumen: | It is an open question whether in multiple myeloma (MM) bone marrow stromal cells contain genomic alterations, which may contribute to the pathogenesis of the disease. We conducted an array-based comparative genomic hybridization (array-CGH) analysis to compare the extent of unbalanced genomic alterations in mesenchymal stem cells from 21 myeloma patients (MM-MSCs) and 12 normal donors (ND-MSCs) after in vitro culture expansion. Whereas ND-MSCs were devoid of genomic imbalances, several non-recurrent chromosomal gains and losses (>1Mb size) were detected in MM-MSCs. Using real-time reverse transcription PCR, we found correlative deregulated expression for five genes encoded in regions for which genomic imbalances were detected using array-CGH. In addition, only MM-MSCs showed a specific pattern of 'hot-spot' regions with discrete (<1Mb) genomic alterations, some of which were confirmed using fluorescence in situ hybridization (FISH). Within MM-MSC samples, unsupervised cluster analysis did not correlate with particular clinicobiological features of MM patients. We also explored whether cytogenetic abnormalities present in myelomatous plasma cells (PCs) were shared by matching MSCs from the same patients using FISH. All MM-MSCs were cytogenetically normal for the tested genomic alterations. Therefore we cannot support a common progenitor for myeloma PCs and MSCs. | URI: | http://hdl.handle.net/10261/61288 | DOI: | 10.1038/leu.2009.65 | Identificadores: | doi: 10.1038/leu.2009.65 issn: 0887-6924 e-issn: 1476-5551 |
Aparece en las colecciones: | (IBMCC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
119
checked on 12-abr-2024
WEB OF SCIENCETM
Citations
107
checked on 29-feb-2024
Page view(s)
295
checked on 23-abr-2024
Download(s)
80
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.