Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/66331
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Specific Roles of Akt iso Forms in Apoptosis and Axon Growth Regulation in Neurons |
Autor: | Díez, Héctor; Garrido Jurado, Juan José CSIC ORCID ; Wandosell, Francisco CSIC ORCID | Fecha de publicación: | 2012 | Editor: | Public Library of Science | Citación: | PLoS ONE (2012) | Resumen: | Akt is a member of the AGC kinase family and consists of three isoforms. As one of the major regulators of the class I PI3 kinase pathway, it has a key role in the control of cell metabolism, growth, and survival. Although it has been extensively studied in the nervous system, we have only a faint knowledge of the specific role of each isoform in differentiated neurons. Here, we have used both cortical and hippocampal neuronal cultures to analyse their function. We characterized the expression and function of Akt isoforms, and some of their substrates along different stages of neuronal development using a specific shRNA approach to elucidate the involvement of each isoform in neuron viability, axon development, and cell signalling. Our results suggest that three Akt isoforms show substantial compensation in many processes. However, the disruption of Akt2 and Akt3 significantly reduced neuron viability and axon length. These changes correlated with a tendency to increase in active caspase 3 and a decrease in the phosphorylation of some elements of the mTORC1 pathway. Indeed, the decrease of Akt2 and more evident the inhibition of Akt3 reduced the expression and phosphorylation of S6. All these data indicate that Akt2 and Akt3 specifically regulate some aspects of apoptosis and cell growth in cultured neurons and may contribute to the understanding of mechanisms of neuron death and pathologies that show deregulated growth. | URI: | http://hdl.handle.net/10261/66331 | DOI: | 10.1371/journal.pone.0032715 | Identificadores: | doi: 10.1371/journal.pone.0032715 issn: 1932-6203 |
Aparece en las colecciones: | (IC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Garrido,JJJ,2012,PlosOne,2012.pdf | 3,38 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
39
checked on 11-abr-2024
SCOPUSTM
Citations
65
checked on 17-abr-2024
WEB OF SCIENCETM
Citations
61
checked on 25-feb-2024
Page view(s)
331
checked on 18-abr-2024
Download(s)
205
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.