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Título: | Direct actions of adrenergic agents on rat anterior pituitary cells |
Autor: | Alarcón, Pilar CSIC; Núñez, Lucía CSIC ORCID; García-Sancho, Javier CSIC ORCID | Fecha de publicación: | 2001 | Editor: | Springer Nature | Citación: | Pflügers Archiv European Journal of Physiology 442(6): 834-841 (2001) | Resumen: | We studied the effects of adrenergic agents on the five main cell types of the rat anterior pituitary by monitoring the changes of the cytosolic free [Ca2+] ([Ca2+]i) in single cells that were identified by multiple sequential primary immunocytochemistry at the end of the Ca2+ measurements. Adrenaline (100 nM) increased [Ca2+]i in 30% of the cells. Responses were most prominent in somatotrophs and corticotrophs (40-65% of the cells responded) whereas the other three cell types, lactotrophs, thyrotrophs and gonadotrophs, gave poorer responses. Selective agonists and antagonists revealed the presence of both α1- and β-adrenergic receptors. α1-Receptors dominated in corticotrophs, β-receptors in somatotrophs. The α1-adrenergic responses increased with culture of the cells. The β-adrenergic responses were mediated by cAMP and consisted of stimulation of Ca2+ entry through L-type voltage-gated channels. Stimulation of α1-receptors released Ca2+ from intracellular stores in corticotrophs and induced cAMP-independent Ca2+ entry in somatotrophs. The effects of α1-agonists were additive with those of the releasing factors growth hormone-releasing hormone (GHRH) and corticotropin releasing factor (CRF) whereas those of the β-agonists were not. Our results suggest that direct effects of plasma catecholamines on AP cells may contribute to the hormonal response to stress. | URI: | http://hdl.handle.net/10261/71605 | DOI: | 10.1007/s004240100622 | Identificadores: | doi: 10.1007/s004240100622 issn: 0031-6768 1432-2013 |
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