Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/73429
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Spatially resolved analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) |
Autor: | Peukert, Manuela; Matros, Andrea; Lattanzio, Giuseppe CSIC ORCID CVN; Kaspar, Stephanie; Abadía Bayona, Javier CSIC ORCID ; Mock, Hans-Peter | Palabras clave: | Barley grains MALDI-MSI sample preparation small molecules tobacco roots |
Fecha de publicación: | feb-2012 | Editor: | John Wiley & Sons | Citación: | Peukert M, Matros A, Lattanzio G, Kaspar S, Abadía J, Mock HP. Spatially resolved analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI). New Phytologist 193 (3): 806-815 (2012) | Resumen: | Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) of tissues provides the means to analyse the spatial distributions of small molecules and proteins within tissues. This imaging technique is commonplace in medicinal and pharmaceutical research, but its application in plant science is very recent. Broader introduction requires specific adaptations for plant tissues. Sample preparation is of paramount importance in order to obtain high-quality spectra providing sufficient spatial resolution for compounds. Optimization is required for sectioning, choice of matrix and means of matrix deposition. Here, we present our current protocols for the detection of small molecules in cryodissected immature barley (Hordeum vulgare) grains and tobacco (Nicotiana tabacum) roots. Examples of MALDI-MSI measurements are provided, and the level of reproducibility across biological replicates is addressed. Furthermore, our approaches for the validation of distribution patterns and for the identification of molecules are described. Finally, we discuss how MALDI-MSI can contribute to applied plant research. | Descripción: | 10 Pags., 6 Figs., 1 Tabl. | Versión del editor: | http://dx.doi.org/10.1111/j.1469-8137.2011.03970.x | URI: | http://hdl.handle.net/10261/73429 | DOI: | 10.1111/j.1469-8137.2011.03970.x | ISSN: | 0028-646X | E-ISSN: | 1469-8137 |
Aparece en las colecciones: | (EEAD) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
mod vers.aut pend.pdf | 20,83 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
69
checked on 12-mar-2024
WEB OF SCIENCETM
Citations
69
checked on 29-feb-2024
Page view(s)
519
checked on 18-mar-2024
Download(s)
243
checked on 18-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.