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Título: | Transcriptional activation by AP‐2α is modulated by the oncogene DEK |
Autor: | Campillos, Mónica; García, Miguel Ángel; Valdivieso Amate, Fernando; Vázquez, Jesús CSIC ORCID CVN | Palabras clave: | AP-2 in vitro Oncoprotein DEK |
Fecha de publicación: | 2003 | Editor: | Oxford University Press | Citación: | Nucleic Acids Research, 2003, Vol. 31, No. 5 1571-1575 | Resumen: | Cell differentiation and development are highly regulated processes at the transcriptional level. One of the main transcription factors that regulate these processes is AP-2alpha, a cell-type specific protein required for vertebrate development and embryogenesis. AP-2alpha also regulates apoptosis and cell-cycle specific events by interacting with the oncogene c-Myc. In searching for novel AP-2alpha- interacting factors, using an affinity chromatography approach, we have observed that oncoprotein DEK interacts with AP-2alpha in vitro. The existence of an interaction between AP-2alpha and DEK in cellular cultures was demonstrated by expression of a tagged AP-2alpha form followed by immunodetection. By transient co-expression experiments using a reporter for APOE promoter activity we have found that DEK stimulates the transactivation activity of AP-2alpha over APOE promoter. Finally, electrophoretic mobility shift assays suggested that DEK enhances the DNA-binding activity of AP-2alpha. Our data suggest a novel cellular function of DEK as a transcriptional co-activator | Versión del editor: | http://dx.doi.org/10.1093/nar/gkg247 | URI: | http://hdl.handle.net/10261/8250 | ISSN: | 0305-1048 | E-ISSN: | 1362-4962 |
Aparece en las colecciones: | (CBM) Artículos |
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