Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/89992
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | On-capillary derivatization and analysis of amino acids in human plasma by capillary electrophoresis with laser-induced fluorescence detection: Application to diagnosis of aminoacidopathies |
Autor: | Veledo, María Teresa CSIC; Frutos, Mercedes de CSIC ORCID; Díez-Masa, José Carlos CSIC ORCID | Fecha de publicación: | 2006 | Editor: | John Wiley & Sons | Citación: | Electrophoresis 27: 3101- 3107 (2006) | Resumen: | A capillary electrophoresis with laser-induced fluorescence detection method for the analysis of free amino acids (AA) in human plasma was developed. A mixture of 16 AA was on-capillary derivatized with 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ) and separated inside the capillary in less than 30 min using 70 mM borax-3.5 mM SDS pH 9.3 as running buffer. Four plasma samples from a healthy donor and patients suffering from phenylketonuria, propionic acidemia, and tyrosinemia type II were studied. Repeatabilities calculated as intra-day RSD (n = 3) values for the AA involved in these aminoacidopathies (glycine, phenylalanine, and tyrosine) were in the range of 0.3 to 1.2% for migration time and 3.7 to 8.2% for peak height. Reproducibilities calculated as inter-day RSD (n = 4) values for the same AA were between 0.7 and 1.4% for migration time and 4.7 and 9.1% for peak height. A fast qualitative analysis allowed the identification of the corresponding disease by comparing the electrophoretic profiles from the patient and the healthy donor and noting the increased level of the specific AA accompanying each individual disease. The results of the quantitative analysis for glycine, phenylalanine, and tyrosine in the plasma samples studied using the developed method showed a good agreement with those provided by the Center of Diagnosis of Molecular Diseases using a standard method for AA analysis. © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | URI: | http://hdl.handle.net/10261/89992 | DOI: | 10.1002/elps.200500781 | Identificadores: | doi: 10.1002/elps.200500781 issn: 0173-0835 e-issn: 1522-2683 |
Aparece en las colecciones: | (IQOG) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
23
checked on 16-mar-2024
WEB OF SCIENCETM
Citations
23
checked on 23-feb-2024
Page view(s)
212
checked on 28-mar-2024
Download(s)
99
checked on 28-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.