Temperature- and H-NS-Dependent Regulation of a Plasmid-Encoded Virulence Operon Expressing Escherichia coli Hemolysin
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Autoría
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Fecha de publicación
2002-09 -
Editorial
American Society for Microbiology -
Cita bibliográfica
Madrid, C., Nieto Liñán, J.M., Paytubi, S., Falconi, M., Gualerzi, C.O.: "Temperature- and H-NS-Dependent Regulation of a Plasmid-Encoded Virulence Operon Expressing Escherichia coli Hemolysin". Journal of Bacteriology. Vol. 184, No. 18, págs. 5058–5066 (2002). ISSN 0021-9193 -
Resumen
Proteins H-NS and Hha form a nucleoprotein complex that modulates expression of the thermoregulated hly operon of Escherichia coli. We have been able to identify two H-NS binding sites in the hly regulatory region. One of them partially overlaps the promoter region (site II), and the other is located about 2 kbp upstream (site I). In contrast, Hha protein did not show any preference for specific sequences. In vitro, temperature influences the affinity of H-NS for a DNA fragment containing both binding sites and H-NS-mediated repression of hly operon transcription. Deletion analysis of the hly regulatory region confirms the relevance of site I for thermoregulation of this operon. We present a model to explain the temperature-modulated repression of the hly operon, based on the experiments reported here and other, preexisting data.
Fichero | Tamaño | Formato |
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Temperature- and H-NS-Dependent.pdf | 870.3Kb | Ver/ | versión editor |
Fichero | Tamaño | Formato |
| Descripción |
---|---|---|---|---|
Temperature- and H-NS-Dependent.pdf | 870.3Kb | Ver/ | versión editor |