Item Details

title

Behavioral aversion to AITC requires both painless and dTRPA1 in Drosophila

author

Mandel, S.

author

Shoaf, M.

author

Braco, J.

author

Silver, W.

author

Johnson, E.

abstract

There has been disagreement over the functional roles of the painless gene product in the detection and subsequent behavioral aversion to the active ingredient in wasabi, allyl isothiocyanate (AITC). Originally, painless was reported to eliminate the behavioral aversion to AITC, although subsequent reports suggested that another trpA homolog, dTRPA1, was responsible for AITC aversion. We re-evaluated the role of the painless gene in the detection of AITC, employing several different behavioral assays. Using the proboscis extension reflex (PER) assay, we observed that AITC did not reduce PER frequencies in painless or dTRPA1 mutants but did in wild-type genotypes. Quantification of food intake showed a significant decline in food consumption in the presence of AITC in wild-type, but not painless mutants.

subject

nociception

Drosophila

TRPA

allyl isothiocyanate

aversion

date

2020-03-09T16:15:46Z (accessioned)

2020-03-09T16:15:46Z (available)

7/3/18 (issued)

identifier

Mandel, S. J., Shoaf, M. L., Braco, J. T., Silver, W. L., & Johnson, E. C. J. (2018). Behavioral aversion to AITC requires both painless and dTRPA1 in Drosophila. Frontiers in neural circuits, 12, 45. (citation)

http://hdl.handle.net/10339/96043 (uri)

identifier

https://doi.org/10.3389/fncir.2018.00045 (doi)

language

en (iso)

publisher

Frontiers

rights

https://creativecommons.org/licenses/by/4.0/ (uri)

source

Frontiers in Neural Circuits

type

Article