Genetic and Structural Analysis of the Bacteroides Conjugative Transposon CTn341
Author
Bacic, Melissa K.; Parker, Anita C.; Stagg, J.; Whitley, H. P.; Wells, W. G.; Jacob, L. A.; Smith, C. J.
Abstract
The genetic structure and functional organization of a Bacteroides conjugative transposon (CTn), CTn341, were determined. CTn341 was originally isolated from a tetracycline-resistant clinical isolate of Bacteroides vulgatus. The element was 51,993 bp long, which included a 5-bp coupling sequence that linked the transposon ends in the circular form. There were 46 genes, and the corresponding gene products fell into three major functional groups: DNA metabolism, regulation and antibiotic resistance, and conjugation. The G C content and codon usage observed in the functional groups suggested that the groups belong to different genetic lineages, indicating that CTn341 is a composite, modular element. Mutational analysis of genes representing the different functional groups provided evidence for the gene assignments and showed that the basic conjugation and excision genes are conserved among Bacteroides spp. A group IIA1 intron, designated B.f.I1, was found to be inserted into the bmhA methylase gene. Reverse transcriptase PCR analysis of CTn341 RNA showed that B.fr.I1 was functional and was spliced out of the bmhA gene. Six related CTn-like elements were found in the genome sequences of Bacteroides fragilis NCTC9343 and Bacteroides thetaiotaomicron VPI5482. The putative elements were similar to CTn341 primarily in the tra and mob regions and in the exc gene, and several appeared to contain intron elements. Our data provide the first reported sequence for a complete Bacteroides CTn, and they should be of considerable benefit to further functional and genetic analyses of antibiotic resistance elements and genome evolution in Bacteroides. Originally published Journal of Bacteriology, Vol. 187, No. 8, Apr 2005
Date
2005-04
Citation:
APA:
Bacic, Melissa K., & Parker, Anita C., & Stagg, J., & Whitley, H. P., & Wells, W. G., & Jacob, L. A., & Smith, C. J.. (April 2005).
Genetic and Structural Analysis of the Bacteroides Conjugative Transposon CTn341.
Journal of Bacteriology,
187(8),
2858-
2869. Retrieved from
http://hdl.handle.net/10342/3201
MLA:
Bacic, Melissa K., and Parker, Anita C., and Stagg, J., and Whitley, H. P., and Wells, W. G., and Jacob, L. A., and Smith, C. J..
"Genetic and Structural Analysis of the Bacteroides Conjugative Transposon CTn341". Journal of Bacteriology.
187:8. (2858-2869),
April 2005.
April 23, 2024.
http://hdl.handle.net/10342/3201.
Chicago:
Bacic, Melissa K. and Parker, Anita C. and Stagg, J. and Whitley, H. P. and Wells, W. G. and Jacob, L. A. and Smith, C. J.,
"Genetic and Structural Analysis of the Bacteroides Conjugative Transposon CTn341," Journal of Bacteriology 187, no.
8 (April 2005),
http://hdl.handle.net/10342/3201 (accessed
April 23, 2024).
AMA:
Bacic, Melissa K., Parker, Anita C., Stagg, J., Whitley, H. P., Wells, W. G., Jacob, L. A., Smith, C. J..
Genetic and Structural Analysis of the Bacteroides Conjugative Transposon CTn341. Journal of Bacteriology.
April 2005;
187(8):
2858-2869.
http://hdl.handle.net/10342/3201. Accessed
April 23, 2024.
Collections
Publisher
East Carolina University