항체 생성세포의 발생기원에 관한 연구(1)

Abstract

Adult rats were injected intravenously with 20mg of horseradish peroxidase. A series of rats were sacrificed at intervals ranging from 1 minute to 24 hours, and spleens were processed for sensitive histochemical reaction to localize exogenous peroxi dase. Methyl green pyronin staining and acid phos• phatase reaction were also applied to them singly or combined with HRP staining. Numerous HRP trapping ceIls were found throughout the red pulp , junction of red and white pulp of the spleen , while in the white pulp, no evidence of HRP positive ce11s were obtained. Lysosome rich ce11s in both of white and red pulp appeared positively by the acid phosphatase reaction, but not a11 of them were positive in HRP staining. In HRP-MGP double stained sections, HRP was localized mainly in the macrophages of the splenic cord and lining ce11s of the blood sinuses. Uptake of exogenous antigen by the splenic macrophages was recognized 1 minute after injection and was increased progressively up to 2 hours. No trace of HRP was observed in the ce11s or in the interce11ular spaces of the white pulp.